eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA-gold affinity interactions

被引：70

作者：

Ibn Sina, Abu Ali ^{[1
]}

Howell, Sidney ^{[1
]}

Carrascosa, Laura G. ^{[1
]}

Rauf, Sakandar ^{[1
]}

Shiddiky, Muhammad J. A. ^{[1
]}

Trau, Matt ^{[1
,2
]}

机构：

[1] Univ Queensland, AIBN, Brisbane, Qld 4072, Australia

[2] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 86期

基金：

澳大利亚研究理事会;

关键词：

SINGLE-STRANDED-DNA; LABEL-FREE; CANCER; ADSORPTION; PROBE; PCR;

D O I：

10.1039/c4cc06732k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a simple electrochemical method referred to as "eMethylsorb" for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.

引用

页码：13153 / 13156

页数：4

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