eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA-gold affinity interactions

被引：70

作者：

Ibn Sina, Abu Ali ^{[1
]}

Howell, Sidney ^{[1
]}

Carrascosa, Laura G. ^{[1
]}

Rauf, Sakandar ^{[1
]}

Shiddiky, Muhammad J. A. ^{[1
]}

Trau, Matt ^{[1
,2
]}

机构：

[1] Univ Queensland, AIBN, Brisbane, Qld 4072, Australia

[2] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 86期

基金：

澳大利亚研究理事会;

关键词：

SINGLE-STRANDED-DNA; LABEL-FREE; CANCER; ADSORPTION; PROBE; PCR;

D O I：

10.1039/c4cc06732k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a simple electrochemical method referred to as "eMethylsorb" for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.

引用

页码：13153 / 13156

页数：4

共 28 条

[1] DNA Electrochemistry as a probe of base pair stacking in A-, B-, and Z-form DNA [J].

Boon, EM ;

Barton, JK .

BIOCONJUGATE CHEMISTRY, 2003, 14 (06) :1140-1147

[2] Sensitive and Label-Free DNA Methylation Detection by Ligation-Mediated Hyperbranched Rolling Circle Amplification [J].

Cao, Anping ;

Zhang, Chun-yang .

ANALYTICAL CHEMISTRY, 2012, 84 (14) :6199-6205

[3] Molecular inversion probe-based SPR biosensing for specific, label-free and real-time detection of regional DNA methylation [J].

Carrascosa, Laura G. ;

Ibn Sina, Abu Ali ;

Palanisamy, Ramkumar ;

Sepulveda, Borja ;

Otte, Marinus A. ;

Rauf, Sakandar ;

Shiddiky, Muhamad J. A. ;

Trau, Matt .

CHEMICAL COMMUNICATIONS, 2014, 50 (27) :3585-3588

[4] Validation of DNA Methylation Biomarkers for Diagnosis of Acute Lymphoblastic Leukemia [J].

Chatterton, Zac ;

Burke, Daniel ;

Emslie, Kerry R. ;

Craig, Jeffery M. ;

Ng, Jane ;

Ashley, David M. ;

Mechinaud, Francoise ;

Saffery, Richard ;

Wong, Nicholas C. .

CLINICAL CHEMISTRY, 2014, 60 (07) :995-1003

[5] Determination of the heterogeneity of DNA methylation by combined bisulfite restriction analysis and capillary electrophoresis with laser-induced fluorescence [J].

Chen, Hua-Chien ;

Chang, Yu-Sun ;

Chen, Shu-Jen ;

Chang, Po-Ling .

JOURNAL OF CHROMATOGRAPHY A, 2012, 1230 :123-129

[6] DNA methylation: Bisulphite modification and analysis [J].

Clark, Susan J. ;

Statham, Aaron ;

Stirzaker, Clare ;

Molloy, Peter L. ;

Frommer, Marianne .

NATURE PROTOCOLS, 2006, 1 (05) :2353-2364

[7] Electrochemical DNA sensors [J].

Drummond, TG ;

Hill, MG ;

Barton, JK .

NATURE BIOTECHNOLOGY, 2003, 21 (10) :1192-1199

[8] Mechanisms of disease: Gene silencing in cancer in association with promoter hypermethylation [J].

Herman, JG ;

Baylin, SB .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 349 (21) :2042-2054

[9] Methylation-specific PCR: A novel PCR assay for methylation status of CpG islands [J].

Herman, JG ;

Graff, JR ;

Myohanen, S ;

Nelkin, BD ;

Baylin, SB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (18) :9821-9826

[10] Characterization of DNA probes immobilized on gold surfaces [J].

Herne, TM ;

Tarlov, MJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (38) :8916-8920

← 1 2 3 →