UV induced autofluorescence spectroscopy in Barrett's esophagus

被引：0

作者：

Padilla-Ybarra, JJ ^{[1
]}

Bourg-Heckly, G ^{[1
]}

A'Amar, O ^{[1
]}

Blais, J ^{[1
]}

Etienne, J ^{[1
]}

Guillemin, F ^{[1
]}

机构：

[1] Ctr Alexis Vautrin, IMAC, CRAN, CNRS D0821, F-54511 Vandoeuvre Nancy, France

来源：

OPTICAL BIOPSIES AND MICROSCOPIC TECHNIQUES II | 1997年 / 3197卷

关键词：

autofluorescence; Barrett's esophagus; spectroscopy; optical biopsy;

D O I：

10.1117/12.297950

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Preliminary results of clinical experiments using UV induced autofluorescence spectroscopy in 23 patients with Barrett's Esophagus are reported in this paper. Excitation wavelengths of 351 nn and 330 nm were used to induce Barrett's mucosa autofluorescence. Autofluorescence acquisition and signal processing were performed using a CP200 Jobin-Yvon system coupled to a flexible three optical fiber sensor. Three distinct emission bands were observed in the measured spectra after normalization according to the backscattered light power. These emissions were attributed to collagen, elastin, NADH and flavin. Fluorescence intensities and ratios between the emission bands were used to discriminate high grade dysplasia and early stage cancer in Barrett's esophagus from normal surrounding tissues. A significant decrease of the overall fluorescence intensity was observed for the Barrett's mucosa compared to the normal esophageal one. Autofluorescence spectral shape was modified. Collagen and elastin contribution was found to decrease going from normal to tumoral tissue.

引用

页码：54 / 59

页数：6