Simple approach for evaluation of matrix effect in the mass spectrometry of synthetic cannabinoids

A simple approach for studying and identifying matrix effect is described. This method for the determination of matrix effect combines the advantages of two most popular traditional methods while eliminating their disadvantages. A postcolumn infusion system was used to observe the MS signal alterations of synthetic cannabinoids: UR-144, XLR-11 and STS-135. Protein precipitation, liquid-liquid extraction and solid phase extraction sample preparation methods were tested. The results of the experiments showed that the discussed method of matrix effect estimation can have practical application in the development of analytical methods. The comparison of the normalized matrix effect profiles can be done even for data obtained over time. Obtained results also indicated that matrix effect was highly dependent on sample preparation. Although similar structure, significant differences were observed for different synthetic cannabinoids.