Preoxygenation of Different Preservation Solutions for Porcine Pancreas Preservation

被引:5
作者
Hackl, F. [1 ]
Stiegler, P. [1 ]
Stadlbauer, V. [1 ]
Schaffellner, S. [1 ]
Iberer, F. [1 ]
Matzi, V. [1 ]
Maier, A. [1 ]
Klemen, H. [1 ]
Smolle-Juettner, F. M. [1 ]
Tscheliessnigg, K. [1 ]
机构
[1] Med Univ Gray, Dept Surg, Div Transplantat Surg, A-8036 Graz, Austria
关键词
COLD-STORAGE METHOD; SUCCESSFUL ISLET ISOLATION; 2-LAYER METHOD; TRANSPLANTATION; OXYGENATION; ISCHEMIA;
D O I
10.1016/j.transproceed.2010.02.071
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Introduction. Organ preservation quality impacts porcine islet cell isolation and transplantation success. Among several preservation methods, the two-layer method is promising, but technically demanding and fails to deliver sufficient oxygen. The use of hyperbaric oxygenation may be an easier, more effective method to supply high partial pressure of oxygen (pO(2)) for organ storage. Therefore, the aim of this study was to test the capability of preoxygenation of various preservation solutions with HBO to maintain high pO(2) levels. Methods. University of Wisconsin (UW), Custodiol, Perfadex, or Celsior solutions were preoxygenated in a pressure chamber. NaCl served as the control. pO(2) levels were measured at defined times. The oxygen storage capability was evaluated by leaving the storage bottles open for 2 minutes. Results. It was feasible to preoxygenate preservation solutions. The best solution to maintain high pO(2) tensions was Perfadex, followed by Celsior, and UW. Discussion. The greater the amount of oxygen in the preservation solution, the more oxygen can be delivered to the preserved pancreas. Further studies on the influence of preoxygenated preservation solutions on the porcine pancreas are warranted to improve organ quality, porcine islet cell isolation, and transplantation success.
引用
收藏
页码:1621 / 1623
页数:3
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