Short RNAs Are Transcribed from Repressed Polycomb Target Genes and Interact with Polycomb Repressive Complex-2

被引：301

作者：

Kanhere, Aditi ^{[1
]}

Viiri, Keijo ^{[1
]}

Araujo, Carla C. ^{[1
]}

Rasaiyaah, Jane ^{[1
]}

Bouwman, Russell D. ^{[1
]}

Whyte, Warren A. ^{[2
,3
]}

Pereira, C. Filipe ^{[4
]}

Brookes, Emily ^{[4
]}

Walker, Kimberly ^{[2
]}

Bell, George W. ^{[2
]}

Pombo, Ana ^{[4
]}

Fisher, Amanda G. ^{[4
]}

Young, Richard A. ^{[2
,3
]}

Jenner, Richard G. ^{[1
]}

机构：

[1] UCL, Div Infect & Immun, London W1T 4JF, England

[2] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[3] MIT, Dept Biol, Cambridge, MA 02141 USA

[4] Univ London Imperial Coll Sci Technol & Med, Ctr Clin Sci, MRC, London W12 0NN, England

来源：

MOLECULAR CELL | 2010年 / 38卷 / 05期

基金：

芬兰科学院;

关键词：

HISTONE H3; DEVELOPMENTAL REGULATORS; POLYMERASE-II; MOUSE; METHYLATION; PLURIPOTENT; RECRUITMENT; MAPS; EZH2;

D O I：

10.1016/j.molcel.2010.03.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polycomb proteins maintain cell identity by repressing the expression of developmental regulators specific for other cell types. Polycomb repressive complex-2 (PRC2) catalyzes trimethylation of histone H3 lysine-27 (H3K27me3). Although repressed, PRC2 targets are generally associated with the transcriptional initiation marker H3K4me3, but the significance of this remains unclear. Here, we identify a class of short RNAs, similar to 50-200 nucleotides in length, transcribed from the 5' end of polycomb target genes in primary T cells and embryonic stem cells. Short RNA transcription is associated with RNA polymerase II and H3K4me3, occurs in the absence of mRNA transcription, and is independent of polycomb activity. Short RNAs form stem-loop structures resembling PRC2 binding sites in Xist, interact with PRC2 through SUZ12, cause gene repression in cis, and are depleted from polycomb target genes activated during cell differentiation. We propose that short RNAs play a role in the association of PRC2 with its target genes.

引用

页码：675 / 688

页数：14

共 33 条

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