Amperometric Determination of Catecholamines by Enzymatic Biosensors in Flow Systems

被引：16
作者：
Josypcuk, Oksana [1 ]
Barek, Jiri [2 ]
Josypcuk, Bohdan [1 ]
机构：
[1] CAS, J Heyrovsky Inst Phys Chem, Vvi, Dolejskova 3, Prague 18223 8, Czech Republic
[2] Charles Univ Prague, Dept Analyt Chem, Fac Sci, UNESCO Lab Environm Electrochem, Hlavova 2030-8, Prague 12843 2, Czech Republic
来源：
ELECTROANALYSIS | 2018年 / 30卷 / 06期
关键词：
Amperometry; Silver amalgam; Flow analysis; Enzymatic biosensor; Catecholamines; SILVER SOLID AMALGAM; SELF-ASSEMBLED MONOLAYER; TUBULAR DETECTOR; INJECTION DETERMINATION; PHENOLIC-COMPOUNDS; TYROSINASE; REACTOR; PHEOCHROMOCYTOMA; NOREPINEPHRINE; INACTIVATION;
D O I：
10.1002/elan.201800078
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Four various biosensors based on flow enzymatic mini-reactors containing one of the two different mesoporous silica powders (SBA-15 or MCM-41) modified by one of the two enzymes (laccase or tyrosinase) were used for the determination of L-DOPA, dopamine, noradrenaline, and adrenaline. Dependences of peak currents on detection potentials, flow rates, injected volumes, reactor volumes, and analyte concentrations were studied and optimized. The silica powder of MCM-41 with covalently bonded laccase (Lac) was found to be the best filling for the detection of catecholamines. Relative current response of such biosensor was: 100% for dopamine, 32.1% for L-DOPA, 26.2% for noradrenaline, and 0.71% for adrenaline. Statistical evaluation of parallel determinations of studied analytes with this biosensor proved its good accuracy (RSD=1.82-5.18%) and sensitivity (5.46-15.5molL(-1)). Life time of the studied biosensors is several months. The practical applicability of Lac-MCM41 biosensor was successfully verified by the determination of dopamine and noradrenaline in medical solutions for infusions.
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收藏
页码：1163 / 1171
页数：9
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