Optimisation of Recombinant Myrosinase Production in Pichia pastoris

被引：14

作者：

Rosenbergova, Zuzana ^{[1
]}

Kantorova, Kristina ^{[1
]}

Simkovic, Martin ^{[2
]}

Breier, Albert ^{[2
]}

Rebros, Martin ^{[1
]}

机构：

[1] Slovak Univ Technol Bratislava, Fac Chem & Food Technol, Inst Biotechnol, Radlinskeho 9, Bratislava 81237, Slovakia

[2] Slovak Univ Technol Bratislava, Fac Chem & Food Technol, Inst Biochem & Microbiol, Radlinskeho 9, Bratislava 81237, Slovakia

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2021年 / 22卷 / 07期

关键词：

myrosinase; Pichia pastoris; fermentation; upscale; Arabidopsis thaliana; HETEROLOGOUS PROTEINS; BRASSICA-NAPUS; EXPRESSION; YEAST; TEMPERATURE; INHIBITION; GENE; FERMENTATION; ARABIDOPSIS; TREHALASE;

D O I：

10.3390/ijms22073677

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Myrosinase is a plant defence enzyme catalysing the hydrolysis of glucosinolates, a group of plant secondary metabolites, to a range of volatile compounds. One of the products, isothiocyanates, proved to have neuroprotective and chemo-preventive properties, making myrosinase a pharmaceutically interesting enzyme. In this work, extracellular expression of TGG1 myrosinase from Arabidopsis thaliana in the Pichia pastoris KM71H (Mut(S)) strain was upscaled to a 3 L laboratory fermenter for the first time. Fermentation conditions (temperature and pH) were optimised, which resulted in a threefold increase in myrosinase productivity compared to unoptimised fermentation conditions. Dry cell weight increased 1.5-fold, reaching 100.5 g/L without additional glycerol feeding. Overall, a specific productivity of 4.1 U/L-medium/h was achieved, which was 102.5-fold higher compared to flask cultivations.

引用

页数：14

共 43 条

[11] PRODUCTION OF MOUSE EPIDERMAL GROWTH-FACTOR IN YEAST - HIGH-LEVEL SECRETION USING PICHIA-PASTORIS STRAINS CONTAINING MULTIPLE GENE COPIES [J].