Graphene Quantum Dots-based Electrochemical Biosensor for Catecholamine Neurotransmitters Detection

被引：71

作者：

Baluta, Sylwia ^{[1
]}

Lesiak, Anna ^{[1
,2
]}

Cabaj, Joanna ^{[1
]}

机构：

[1] Wroclaw Univ Sci & Technol, Fac Chem, Wybrzeze Wyspianskiego 27, PL-50370 Wroclaw, Poland

[2] Wroclaw Univ Sci & Technol, Fac Expt Phys, Wybrzeze Wyspianskiego 27, PL-50370 Wroclaw, Poland

来源：

ELECTROANALYSIS | 2018年 / 30卷 / 08期

关键词：

cyclic voltammetry; electrochemical biosensor; epinephrine; GQDs; laccase; GLASSY-CARBON ELECTRODE; ASCORBIC-ACID; URIC-ACID; SENSITIVE DETECTION; EPINEPHRINE; SENSOR; DOPAMINE; NANOPARTICLES; LACCASE; NANOCOMPOSITE;

D O I：

10.1002/elan.201700825

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An useful electrochemical sensing approach was developed for epinephrine (EP) detection based on graphene quantum dots (GQDs) and laccase modified glassy carbon electrodes (GC). The miniature GC biosensor was designed and constructed via the immobilization of laccase in an electroactive layer of the electrode coated with carbon nanoparticles. This sensing arrangement utilized the catalytic oxidation of EP to epinephrine quinone. The detection process was based on the oxidation of catecholamine in the presence of the enzyme - laccase. With the optimized conditions, the analytical performance demonstrated a high degree of sensitivity -2.9AmM(-1)cm(-2), selectivity in a broad linear range (1-120x10(-6)M) with detection limit of 83nM. Moreover, the method was successfully applied for EP determination in labeled pharmacological samples.

引用

页码：1773 / 1782

页数：10

共 71 条

[61] Electrochemical characterization of poly(fuchsine acid) modified glassy carbon electrode and its application for simultaneous determination of ascorbic acid, epinephrine and uric acid [J].

Taei, M. ;

Hasanpour, F. ;

Tavakkoli, N. ;

Bahrameian, M. .

JOURNAL OF MOLECULAR LIQUIDS, 2015, 211 :353-362

[62] ELECTROCATALYSIS OF A CATHODIC OXYGEN REDUCTION BY LACCASE [J].

TARASEVICH, MR ;

YAROPOLOV, AI ;

BOGDANOVSKAYA, VA ;

VARFOLOMEEV, SD .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1979, 6 (03) :393-403

[63] Applications of nanostructured Pt-Au hybrid film for the simultaneous determination of catecholamines in the presence of ascorbic acid [J].

Thiagarajan, Soundappan ;

Chen, Shen-Ming .

JOURNAL OF SOLID STATE ELECTROCHEMISTRY, 2009, 13 (03) :445-453

[64] Palladium nanoparticles modified electrode for the selective detection of catecholamine neurotransmitters in presence of ascorbic acid [J].

Thiagarajan, Soundappan ;

Yang, Rong-Feng ;

Chen, Shen-Ming .

BIOELECTROCHEMISTRY, 2009, 75 (02) :163-169

[65] Determination of standard rate constants for electrochemical irreversible processes from linear sweep voltammograms [J].

Velasco, JG .

ELECTROANALYSIS, 1997, 9 (11) :880-882

[66] A unique turn-off fluorescent strategy for sensing dopamine based on formed polydopamine (pDA) using graphene quantum dots (GQDs) as fluorescent probe [J].

Weng, Shaohuang ;

Liang, Dong ;

Qiu, Huazhang ;

Liu, Zongen ;

Lin, Zhen ;

Zheng, Zongfu ;

Liu, Ailin ;

Chen, Wei ;

Lin, Xinhua .

SENSORS AND ACTUATORS B-CHEMICAL, 2015, 221 :7-14

[67] Epinephrine sensing at nanostructured Au electrode and determination its oxidative metabolism [J].

Wierzbicka, Ewa ;

Szultka-Mlynska, Malgorzata ;

Buszewski, Boguslaw ;

Sulka, Grzegorz D. .

SENSORS AND ACTUATORS B-CHEMICAL, 2016, 237 :206-215

[68] Determination of Lysozyme by Graphene Oxide-Polyethylene Glycol-Based Fluorescence Resonance Energy Transfer [J].

Xie, Yali ;

An, Jianxin ;

Shi, Pengzhi ;

Ye, Nengsheng .

ANALYTICAL LETTERS, 2017, 50 (01) :148-160

[69] Optimal parameters for laccase-mediated destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels [J].

Yang, Jie ;

Yang, Xiaodan ;

Ye, Xiuyun ;

Lin, Juan .

DATA IN BRIEF, 2016, 7 :1-7

[70] The nano-Au self-assembled glassy carbon electrode for selective determination of epinephrine in the presence of ascorbic acid [J].

Yang, Zhousheng ;

Hu, Guangzhi ;

Chen, Xi ;

Zhao, Jun ;

Zhao, Guangchao .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2007, 54 (02) :230-235

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