Graphene Quantum Dots-based Electrochemical Biosensor for Catecholamine Neurotransmitters Detection

被引：71

作者：

Baluta, Sylwia ^{[1
]}

Lesiak, Anna ^{[1
,2
]}

Cabaj, Joanna ^{[1
]}

机构：

[1] Wroclaw Univ Sci & Technol, Fac Chem, Wybrzeze Wyspianskiego 27, PL-50370 Wroclaw, Poland

[2] Wroclaw Univ Sci & Technol, Fac Expt Phys, Wybrzeze Wyspianskiego 27, PL-50370 Wroclaw, Poland

来源：

ELECTROANALYSIS | 2018年 / 30卷 / 08期

关键词：

cyclic voltammetry; electrochemical biosensor; epinephrine; GQDs; laccase; GLASSY-CARBON ELECTRODE; ASCORBIC-ACID; URIC-ACID; SENSITIVE DETECTION; EPINEPHRINE; SENSOR; DOPAMINE; NANOPARTICLES; LACCASE; NANOCOMPOSITE;

D O I：

10.1002/elan.201700825

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An useful electrochemical sensing approach was developed for epinephrine (EP) detection based on graphene quantum dots (GQDs) and laccase modified glassy carbon electrodes (GC). The miniature GC biosensor was designed and constructed via the immobilization of laccase in an electroactive layer of the electrode coated with carbon nanoparticles. This sensing arrangement utilized the catalytic oxidation of EP to epinephrine quinone. The detection process was based on the oxidation of catecholamine in the presence of the enzyme - laccase. With the optimized conditions, the analytical performance demonstrated a high degree of sensitivity -2.9AmM(-1)cm(-2), selectivity in a broad linear range (1-120x10(-6)M) with detection limit of 83nM. Moreover, the method was successfully applied for EP determination in labeled pharmacological samples.

引用

页码：1773 / 1782

页数：10

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