NMR Characterization of the DNA-binding Domain of Arabidopsis thaliana Telomere Repeat Factor

被引：0

作者：

Lee, Inhwan ^{[1
]}

Kim, Heeyoun ^{[1
]}

Ko, Yoon-Joo ^{[2
]}

Lee, Weontae ^{[1
]}

机构：

[1] Yonsei Univ, Coll Life Sci & Biotechnol, Dept Biochem, Seoul 120749, South Korea

[2] Seoul Natl Univ, Natl Ctr Interuniv Res Facil, Seoul 151747, South Korea

来源：

BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2016年 / 37卷 / 04期

关键词：

Arabidopsis thaliana; Telomere repeat factor (AtTRF); DNA-binding motif; Nuclear magnetic resonance; REVEALS UNIQUE FEATURES; IN-VITRO; PROTEIN; SEQUENCE; TRF1; FAMILY; GENE;

D O I：

10.1002/bkcs.10708

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Telomere repeat factor (AtTRF) derived from Arabidopsis thaliana contains a myb-like domain that binds to double-stranded telomeric DNA. We cloned the myb-like domain of AtTRF (AtTRFmyb ) into a pET-15b vector and expressed the protein in Escherichia coli. AtTRFmyb was purified using Ni-affinity chromatography. DNA-binding mode has been examined by electrophoretic mobility shift assay (EMSA). Fluorescence-quenching experiments determined a K-d value of 6.62 nM between AtTRFmyb and plant telomeric DNA. Data from nuclear magnetic resonance (NMR) spectroscopy together with TALOS+ program provide the secondary structures of AtTRFmyb , suggesting that AtTRFmyb binds its plant telomere DNA with three -helices of a DNA-binding motif.

引用

页码：485 / 489

页数：5

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