NMR Characterization of the DNA-binding Domain of Arabidopsis thaliana Telomere Repeat Factor

被引：0
作者：
Lee, Inhwan [1 ]
Kim, Heeyoun [1 ]
Ko, Yoon-Joo [2 ]
Lee, Weontae [1 ]
机构：
[1] Yonsei Univ, Coll Life Sci & Biotechnol, Dept Biochem, Seoul 120749, South Korea
[2] Seoul Natl Univ, Natl Ctr Interuniv Res Facil, Seoul 151747, South Korea
来源：
BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2016年 / 37卷 / 04期
关键词：
Arabidopsis thaliana; Telomere repeat factor (AtTRF); DNA-binding motif; Nuclear magnetic resonance; REVEALS UNIQUE FEATURES; IN-VITRO; PROTEIN; SEQUENCE; TRF1; FAMILY; GENE;
D O I：
10.1002/bkcs.10708
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Telomere repeat factor (AtTRF) derived from Arabidopsis thaliana contains a myb-like domain that binds to double-stranded telomeric DNA. We cloned the myb-like domain of AtTRF (AtTRFmyb ) into a pET-15b vector and expressed the protein in Escherichia coli. AtTRFmyb was purified using Ni-affinity chromatography. DNA-binding mode has been examined by electrophoretic mobility shift assay (EMSA). Fluorescence-quenching experiments determined a K-d value of 6.62 nM between AtTRFmyb and plant telomeric DNA. Data from nuclear magnetic resonance (NMR) spectroscopy together with TALOS+ program provide the secondary structures of AtTRFmyb , suggesting that AtTRFmyb binds its plant telomere DNA with three -helices of a DNA-binding motif.
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收藏
页码：485 / 489
页数：5
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