Electrospray-assisted characterization and deposition of chlorosomes to fabricate a biomimetic light-harvesting device

被引:46
作者
Modesto-Lopez, Luis B. [1 ]
Thimsen, Elijah J. [1 ]
Collins, Aaron M. [2 ,3 ]
Blankenship, Robert E. [2 ,3 ]
Biswas, Pratim [1 ]
机构
[1] Washington Univ, Dept Energy Environm & Chem Engn, Aerosol & Air Qual Res Lab, St Louis, MO 63130 USA
[2] Washington Univ, Dept Chem, St Louis, MO 63130 USA
[3] Washington Univ, Dept Biol, St Louis, MO 63130 USA
关键词
BACTERIUM CHLOROFLEXUS-AURANTIACUS; GREEN PHOTOSYNTHETIC BACTERIA; SENSITIZED SOLAR-CELLS; ENERGY-TRANSFER; CHLOROBIUM-TEPIDUM; MORPHOLOGY; COMPLEXES; PERFORMANCE; MOBILITY; VIRUSES;
D O I
10.1039/b914758f
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Photosynthesis is an efficient process by which solar energy is converted into chemical energy. Green photosynthetic bacteria such as Chloroflexus aurantiacus have supramolecular antenna complexes called chlorosomes attached to their cytoplasmic membrane that increase the cross section for light absorption even in low-light conditions. Self-assembled bacteriochlorophyll pigments in the chlorosome interior play a key role in the efficient transfer and funneling of the harvested energy. In this work it was demonstrated that chlorosomes can be rapidly and precisely size-characterized online in real time using an electrospray-assisted mobility-based technique. Chlorosomes were electrospray-deposited onto TiO2 nanostructured films with columnar morphology to fabricate a novel biomimetic device to overcome the solvent compatibility issues associated with biological particles and synthetic dyes. The assembled unit retained the viability of the chlorosomes, and the harvesting of sunlight over a broader range of wavelengths was demonstrated. It was shown that the presence of chlorosomes in the biomimetic device had a 30-fold increase in photocurrent.
引用
收藏
页码:216 / 222
页数:7
相关论文
共 35 条
[1]   Nano ES GEMMA and PDMA, new tools for the analysis of nanobioparticles - Protein complexes, lipoparticles, and viruses [J].
Allmaier, Guenter ;
Laschober, Christian ;
Szymanski, Wladyslaw W. .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2008, 19 (08) :1062-1068
[2]   Solid-state dye-sensitized mesoporous TiO2 solar cells with high photon-to-electron conversion efficiencies [J].
Bach, U ;
Lupo, D ;
Comte, P ;
Moser, JE ;
Weissörtel, F ;
Salbeck, J ;
Spreitzer, H ;
Grätzel, M .
NATURE, 1998, 395 (6702) :583-585
[3]   High-performance dye-sensitized solar cells based on solvent-free electrolytes produced from eutectic melts [J].
Bai, Yu ;
Cao, Yiming ;
Zhang, Jing ;
Wang, Mingkui ;
Li, Renzhi ;
Wang, Peng ;
Zakeeruddin, Shaik M. ;
Graetzel, Michael .
NATURE MATERIALS, 2008, 7 (08) :626-630
[4]   REVEALING THE BLUEPRINT OF PHOTOSYNTHESIS [J].
BARBER, J ;
ANDERSSON, B .
NATURE, 1994, 370 (6484) :31-34
[5]   An obligately photosynthetic bacterial anaerobe from a deep-sea hydrothermal vent [J].
Beatty, JT ;
Overmann, J ;
Lince, MT ;
Manske, AK ;
Lang, AS ;
Blankenship, RE ;
Van Dover, CL ;
Martinson, TA ;
Plumley, FG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (26) :9306-9310
[6]  
Blankenship R.E., 1995, Anoxygenic photosynthetic bacteria, P399, DOI DOI 10.1007/0-306-47954-0
[7]   FORSTER ENERGY-TRANSFER IN CHLOROSOMES OF GREEN PHOTOSYNTHETIC BACTERIA [J].
CAUSGROVE, TP ;
BRUNE, DC ;
BLANKENSHIP, RE .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1992, 15 (1-2) :171-179
[8]  
CAUSGROVE TP, 1990, PHOTOSYNTH RES, V26, P39, DOI 10.1007/BF00048975
[9]   ISOLATION AND CHARACTERIZATION OF CYTOPLASMIC MEMBRANES AND CHLOROSOMES FROM THE GREEN BACTERIUM CHLOROFLEXUS-AURANTIACUS [J].
FEICK, RG ;
FITZPATRICK, M ;
FULLER, RC .
JOURNAL OF BACTERIOLOGY, 1982, 150 (02) :905-915
[10]   Antenna size dependent exciton dynamics in the chlorosomal antenna of the green bacterium Chloroflexus aurantiacus [J].
Fetisova, Z ;
Freiberg, A ;
Novoderezhkin, V ;
Taisova, A ;
Timpmann, K .
FEBS LETTERS, 1996, 383 (03) :233-236