Stimulation of Cell Proliferation and Expression of Matrixmetalloproteinase-1 and Interluekin-8 Genes in Dermal Fibroblasts by Copper

被引：69
作者：
Philips, Neena [1 ]
Hwang, Hyeondo [2 ]
Chauhan, Sagar [1 ]
Leonardi, Donna [2 ]
Gonzalez, Salvador [3 ,4 ]
机构：
[1] Fairleigh Dickinson Univ, Sch Nat Sci, Teaneck, NJ 07666 USA
[2] Bergen Acad, Hackensack, NJ USA
[3] Ind Farmaceut Cantabria, Madrid, Spain
[4] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA
来源：
CONNECTIVE TISSUE RESEARCH | 2010年 / 51卷 / 03期
关键词：
Copper; Matrixmetalloprotienase; Interleukin-8; Fibroblasts; Wound Healing; Aging; HUMAN ENDOTHELIAL-CELLS; MATRIX METALLOPROTEINASES; COMPLEX GLYCYL-L-HISTIDYL-L-LYSINE-CU2+; MELANOMA-CELLS; INTERLEUKIN-8; WOUNDS; GROWTH; TETRATHIOMOLYBDATE; MODULATION; RESPONSES;
D O I：
10.3109/03008200903288431
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Copper is essential to wound healing as well as a widespread environmental pollutant, with skin aging potential. Wound healing and skin aging are facilitated by matrixmetalloproteinases (MMP), which remodel the extracellular matrix, and interleukin-8 (IL-8), linked with copper. This research investigated the mechanism to copper's role in wound healing or skin aging by regulation of MMP-1 and IL-8 genes. It examined the dose-responsive effects of copper on MMP-1, -2, and -9 activities; MMP-1 and IL-8 gene regulation at protein, mRNA, and promoter levels; tissue inhibitor of matrixmetalloproteinases-1 (TIMP-1) expression; and cell proliferation. Copper stimulated cell proliferation and the expression of MMP-1 and IL-8 genes at the protein, mRNA, and promoter levels, indicating transcriptional regulation, without significantly altering TIMP-1. The research suggests that copper facilitates wound healing as well as skin aging via the induction of MMP-1 expression, with limiting MMP effect at the higher concentrations through enhanced IL-8 expression, which favors extracellular matrix deposition.</.
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收藏
页码：224 / 229
页数：6
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