The Use of Infrared Thermography as a Novel Approach for Real-Time Validation of PCR Thermocyclers

被引：2
|
作者：
Gronlund, Hugo Ahlm [1 ]
Lofstrom, Charlotta [1 ]
Helleskov, Jens Bue [2 ]
Hoorfar, Jeffrey [1 ]
机构：
[1] Tech Univ Denmark, Natl Food Inst, DK-2860 Soborg, Denmark
[2] Tryel, DK-2765 Smorum, Denmark
来源：
FOOD ANALYTICAL METHODS | 2010年 / 3卷 / 02期
关键词：
PCR; Thermocycler; Thermal Cycler; Infrared Thermography; Validation; PERFORMANCE; CYCLERS;
D O I：
10.1007/s12161-009-9103-2
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Validation of PCR thermocycler performance is crucial to obtain reliable results. In this study, infrared (IR) thermography was evaluated as a novel validation tool. After stabilisation, no significant difference in the temperatures recorded using thermography and a reference block-based system was found. By employing IR thermography, information about the length of the time until temperature stabilisation in the sample could be obtained. This study shows the potential of using IR thermography for validation of thermocyclers.
引用
收藏
页码：116 / 119
页数：4
相关论文
共 50 条
  • [21] THE USE OF PCR AND REAL-TIME PCR FOR QUALITATIVE AND QUANTITATIVE DETERMINATION OF POULTRY AND CHICKEN MEALS
    Natonek-Wisniewska, Malgorzata
    Krzyscin, Piotr
    ANNALS OF ANIMAL SCIENCE, 2016, 16 (03): : 731 - 741
  • [22] Suggested guidelines for validation of real-time PCR assays in veterinary diagnostic laboratories
    Toohey-Kurth, Kathy
    Reising, Monica M.
    Tallmadge, Rebecca L.
    Goodman, Laura B.
    Bai, Jianfa
    Bolin, Steven R.
    Pedersen, Janice C.
    Bounpheng, Mangkey A.
    Pogranichniy, Roman M.
    Christopher-Hennings, Jane
    Killian, Mary Lea
    Mulrooney, Donna M.
    Maes, Roger
    Singh, Shri
    Crossley, Beate M.
    JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2020, 32 (06) : 802 - 814
  • [23] Development and validation of a real-time PCR specific for the leukotoxin gene of Bibersteinia trehalosi
    Killion, Halcyon J.
    Edwards, William
    Jennings-Gaines, Jessica
    Wood, Mary
    Fox, Karen
    Sondgeroth, Kerry
    JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2018, 30 (04) : 589 - 592
  • [24] Selection and validation of garlic reference genes for quantitative real-time PCR normalization
    Liu, Min
    Wu, Zhen
    Jiang, Fangling
    PLANT CELL TISSUE AND ORGAN CULTURE, 2015, 122 (02) : 435 - 444
  • [25] Validation of a commercial real-time PCR kit for specific and sensitive detection of Pseudorabies
    Pol, Francoise
    Deblanc, Celine
    Oger, Aurelie
    Le Dimna, Mireille
    Simon, Gaelle
    Le Potier, Marie-Frederique
    JOURNAL OF VIROLOGICAL METHODS, 2013, 187 (02) : 421 - 423
  • [26] Selection and validation of garlic reference genes for quantitative real-time PCR normalization
    Min Liu
    Zhen Wu
    Fangling Jiang
    Plant Cell, Tissue and Organ Culture (PCTOC), 2015, 122 : 435 - 444
  • [27] Validation of real-time PCR methods for the quantification of transgenic contaminations in rape seed
    Zeitler, R
    Pietsch, M
    Waiblinger, HU
    EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2002, 214 (04) : 346 - 351
  • [28] Real-time PCR in microfluidic devices
    Becker, Holger
    Hlawatsch, Nadine
    Klemm, Richard
    Moche, Christian
    Hansen-Hagge, Thomas
    Gaertner, Claudia
    MICROFLUIDICS, BIOMEMS, AND MEDICAL MICROSYSTEMS XII, 2014, 8976
  • [29] Reference genes in real-time PCR
    Bartłomiej Kozera
    Marcin Rapacz
    Journal of Applied Genetics, 2013, 54 : 391 - 406
  • [30] Reference genes in real-time PCR
    Kozera, Bartomiej
    Rapacz, Marcin
    JOURNAL OF APPLIED GENETICS, 2013, 54 (04) : 391 - 406
12345