Observation of the resting and pulsed states of cytochrome c oxidase in electrode-supported lipid bilayer membranes

被引：32

作者：

Burgess, JD ^{[1
]}

Rhoten, MC ^{[1
]}

Hawkridge, FH ^{[1
]}

机构：

[1] Virginia Commonwealth Univ, Dept Chem, Richmond, VA 23284 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1998年 / 120卷 / 18期

关键词：

D O I：

10.1021/ja974275z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Cytochrome c oxidase immobilized in lipid bilayer membranes on gold electrodes mediates electron transfer between reduced cytochrome c in solution and the electrode. Under flow conditions, fixed potential amperometry of anaerobic solutions of reduced cytochrome c shows changes in the rate of enzyme mediated electron transfer from cytochrome c to oxidase modified electrodes. These data are consistent with literature that describes both activation of the resting oxidase state to the pulsed oxidase state and subsequent decay of the pulsed oxidase state to the resting oxidase state.

引用

页码：4488 / 4491

页数：4

共 28 条

[1] OXYGEN PULSED CYTOCHROME-C OXIDASE - FUNCTIONAL PROPERTIES AND CATALYTIC RELEVANCE [J].

ANTONINI, E ;

BRUNORI, M ;

COLOSIMO, A ;

GREENWOOD, C ;

WILSON, MT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (08) :3128-3132

[2] PULSED CYTOCHROME-C OXIDASE [J].

ANTONINI, G ;

BRUNORI, M ;

COLOSIMO, A ;

MALATESTA, F ;

SARTI, P .

JOURNAL OF INORGANIC BIOCHEMISTRY, 1985, 23 (3-4) :289-293

[3] OXYGEN ACTIVATION AND THE CONSERVATION OF ENERGY IN CELL RESPIRATION [J].

BABCOCK, GT ;

WIKSTROM, M .

NATURE, 1992, 356 (6367) :301-309

[4]

BLAIR DF, 1986, J BIOL CHEM, V261, P1524

[5] FUNCTIONAL STUDIES ON CROSSLINKED BOVINE CYTOCHROME-C OXIDASE [J].

BONAVENTURA, C ;

BONAVENTURA, J ;

BRUNORI, M ;

WILSON, M .

FEBS LETTERS, 1978, 85 (01) :30-34

[6] PULSED CYTOCHROME-OXIDASE MAY BE PRODUCED WITHOUT THE ADVENT OF DIOXYGEN [J].

BRUNORI, M ;

COLOSIMO, A ;

SARTI, P ;

ANTONINI, E ;

WILSON, MT .

FEBS LETTERS, 1981, 126 (02) :195-198

[7]

BRUNORI M, 1979, J BIOL CHEM, V254, P769

[8] CYTOCHROME-OXIDASE [J].

BRUNORI, M ;

WILSON, MT .

TRENDS IN BIOCHEMICAL SCIENCES, 1982, 7 (08) :295-299

[9] Octadecyl mercaptan sub-monolayers on silver electrodeposited on gold quartz crystal microbalance electrodes [J].

Burgess, JD ;

Hawkridge, FM .

LANGMUIR, 1997, 13 (14) :3781-3786

[10]

BURGESS JD, IN PRESS LANGMUIR

← 1 2 3 →