Isolation and Characterization of a Novel Small Antifungal Peptide from Bacillus megaterium D4 Isolated from the Dung of Wild Plateau Yak in China

被引：2
作者：
Chen, Liang [1 ]
Chen, Wuling [2 ]
机构：
[1] Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Henan, Peoples R China
[2] NW Univ Xian, Coll Life Sci, Xian 710069, Shaanxi, Peoples R China
来源：
PROTEIN AND PEPTIDE LETTERS | 2010年 / 17卷 / 04期
关键词：
Antifungal peptide; Bacillus megaterium; Purification; Thermo stability; Yak dung; BACILLOMYCIN-L; SUBTILIS; BIOCONTROL; LIPOPEPTIDES; PURIFICATION; ANTIBIOTICS; PSEUDOMONAS; SURFACTIN; STRAINS; BANANA;
D O I：
10.2174/092986610790963627
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel small antifungal peptide-producing strain D4 was isolated from the dung of wild plateau yak and identified as Bacillus megaterium. The purification procedure of peptide consisted of acid precipitation, methanol extract and C-18 reverse-phase chromatography. The amino acid composition of peptide YP differed from those of antifungal peptides which have been reported to date. Peptide YP was proved to be a novel small antifungal cyclic peptide. It exhibited strong inhibitory activity against many phytopathogenic fungi and had a wild range of thermo stability and pH stability and was not susceptible to all proteases tested.
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页码：542 / 546
页数：5
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