Analysis of peptides by MALDI-TOF mass spectrometry

被引：0

作者：

Sedo, O ^{[1
]}

Havel, J ^{[1
]}

机构：

[1] Masaryk Univ, Fac Sci, Dept Analyt Chem, CS-61137 Brno, Czech Republic

来源：

CHEMICKE LISTY | 2003年 / 97卷 / 02期

关键词：

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The MALDI-TOF MS method can be used for the exact determination of molecular weight of peptides. Detailed study of fragmentation of metastable ions after leaving the ion source (post-source decay, PSD) makes it possible to determine also the sequence of amino acids in peptides. If complete primary structure cannot be reliably determined due to the complexity of PSD spectra, the missing part of the sequence can be found in Internet bases. Because of a simple preparation of samples and short analysis times, MALDI-TOF MS is an extraordinarily suitable method for checking purity of peptide preparations. It can be also used for study of peptide derivatization.

引用

页码：109 / 113

页数：5

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