Elutability of proteins from aluminum-containing vaccine adjuvants by treatment with surfactants

被引：31
|
作者：
Rinella, JV
Workman, RF
Hermodson, MA
White, JL
Hem, SL [1 ]
机构：
[1] Purdue Univ, Dept Ind & Phys Pharm, W Lafayette, IN 47907 USA
[2] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
[3] Purdue Univ, Dept Biochem, W Lafayette, IN 47907 USA
[4] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA
来源：
JOURNAL OF COLLOID AND INTERFACE SCIENCE | 1998年 / 197卷 / 01期
关键词：
elutability; aluminum hydroxide adjuvant; aluminum phosphate adjuvant; surfactant-induced elution; desorption of preadsorbed proteins; solubilization; micellar solubilization; electrostatic repulsion;
D O I：
10.1006/jcis.1997.5230
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The elutability of proteins from adjuvants in model vaccines composed of ovalbumin adsorbed by aluminum hydroxide adjuvant or lysozyme adsorbed by aluminum phosphate adjuvant following treatment with surfactant solutions was studied. Nonionic (Triton X-100, lauryl maltoside), zwitterionic (lauryl sulfobetaine), anionic (sodium dodecyl sulfate), and cationic (cetylpyridinium chloride, dodecyltrimethylammonium chloride) surfactants were investigated. Cetylpyridinium chloride produced the greatest degree of elution (60%) of ovalbumin from aluminum hydroxide adjuvant. Sodium dodecyl sulfate completely eluted lysozyme from aluminum phosphate adjuvant. The effectiveness of surfactants in removing preadsorbed proteins was directly related to their ability to denature the protein. Micellar solubilization and electrostatic repulsion may also contribute to desorption. (C) 1998 Academic Press.
引用
收藏
页码：48 / 56
页数：9
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