Preoperative interleukin-6 production by mononuclear blood cells predicts survival after radical surgery for colorectal carcinoma

被引:18
作者
Clinchy, Birgitta [1 ]
Fransson, Annelie
Druvefors, Bengt
Hellsten, Anna
Hakansson, Annika
Gustafsson, Bertil
Sjodahl, Rune
Hakansson, Leif
机构
[1] Linkoping Univ Hosp, Div Clin Tumorimmunol, S-58185 Linkoping, Sweden
[2] Linkoping Univ Hosp, Dept Oncol, S-58185 Linkoping, Sweden
[3] Linkoping Univ Hosp, Dept Surg, S-58185 Linkoping, Sweden
[4] Linkoping Univ Hosp, Dept Pathol & Cytol, S-58185 Linkoping, Sweden
关键词
colorectal carcinoma; interleukin-6; prediction; preoperative; radical resection; survival;
D O I
10.1002/cncr.22623
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
BACKGROUND. Colorectal cancer is one of the most common forms of cancer in the Western world. Staging based on histopathology is currently the most accurate predictor of outcome after surgery. Colorectal cancer is curable if treated at an early stage (stage I-III). However, for tumors in stages II and III there is a great need for tests giving more accurate prognostic information defining the patient population in need of closer follow-up and/or adjuvant therapy. Furthermore, tests that provide prognostic information preoperatively could provide a guide both for preoperative oncologic treatment and the surgical procedure. METHODS. Peripheral blood mononuclear cells (PBMCs) were isolated preoperatively, within a week before primary surgery from 39 patients undergoing surgery for colorectal cancer. The PBMCs were cultured in vitro for 24 hours in the presence of autologous serum and lipopolysaccharide (LPS). Interleukin-6 (IL-6) production was measured with enzyme-linked immunosorbent assay (ELISA). Staging based on histopathology was performed in all patients. Patients were followed for at least 54 months. RESULTS. A production of >5000 pg/mL of IL-6 identified colorectal cancer patients with a poor prognosis. Eight out of 13 patients with >5000 pg/mL IL-6 died from cancer within the follow-up period, whereas no cancer-related deaths were recorded among 21 patients with 5000 pg/mL IL-6 or less. A multivariate Cox regression analysis, stratified for T- and N-stage, identified IL-6 production as an independent prognostic factor. CONCLUSIONS. IL-6 production in vitro by PBMC can predict survival after radical surgery for colorectal cancer.
引用
收藏
页码:1742 / 1749
页数:8
相关论文
共 37 条
[1]  
ALKIRA S, 1992, SEMIN CANCER BIOL, V3, P17
[2]   TLR4 mutations are associated with endotoxin hyporesponsiveness in humans [J].
Arbour, NC ;
Lorenz, E ;
Schutte, BC ;
Zabner, J ;
Kline, JN ;
Jones, M ;
Frees, K ;
Watt, JL ;
Schwartz, DA .
NATURE GENETICS, 2000, 25 (02) :187-+
[3]   Interleukin-6 blood level is associated with circulating carcinoembryonic antigen and prognosis in patients with colorectal cancer [J].
Belluco, C ;
Nitti, D ;
Frantz, M ;
Toppan, P ;
Basso, D ;
Plebani, M ;
Lise, M ;
Jessup, JM .
ANNALS OF SURGICAL ONCOLOGY, 2000, 7 (02) :133-138
[4]   Prognostic significance of immunological evaluation in colorectal cancer [J].
Berghella, AM ;
Pellegrini, P ;
DelBeato, T ;
Maccarone, D ;
Adorno, D ;
Casciani, CU .
CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS, 1996, 11 (06) :355-361
[5]   Gene expression profiling for molecular staging and prognosis prediction in colorectal cancer [J].
Bustin, SA ;
Dorudi, S .
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2004, 4 (05) :599-607
[6]   Evaluation of a cumulative prognostic score based on the systemic inflammatory response in patients undergoing potentially curative surgery for colorectal cancer [J].
Canna, K ;
McMillan, DC ;
McKee, RF ;
McNicol, AM ;
Horgan, PG ;
McArdle, CS .
BRITISH JOURNAL OF CANCER, 2004, 90 (09) :1707-1709
[7]   Serum interleukin-6 levels reflect the disease status of colorectal cancer [J].
Chung, YC ;
Chang, YF .
JOURNAL OF SURGICAL ONCOLOGY, 2003, 83 (04) :222-226
[8]   Serum C-reactive protein correlates with survival in colorectal cancer patients but is not an independent prognostic indicator [J].
Chung, YC ;
Chang, YF .
EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY, 2003, 15 (04) :369-373
[9]   Interleukin-6 serum level correlates with survival in advanced gastrointestinal cancer patients but is not an independent prognostic indicator [J].
De Vita, F ;
Romano, C ;
Orditura, M ;
Galizia, G ;
Martinelli, E ;
Lieto, E ;
Catalano, G .
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 2001, 21 (01) :45-52
[10]   Cytokines in pancreatic carcinoma - Correlation with phenotypic characteristics and prognosis [J].
Ebrahimi, B ;
Tucker, SL ;
Li, DH ;
Abbruzzese, JL ;
Kurzrock, R .
CANCER, 2004, 101 (12) :2727-2736