Hydroxylamine oxidation in heterotrophic nitrate-reducing soil bacteria and purification of a hydroxylamine-cytochrome c oxidoreductase from a Pseudomonas species

被引:31
作者
Wehrfritz, JM [1 ]
Carter, JP [1 ]
Spiro, S [1 ]
Richardson, DJ [1 ]
机构
[1] UNIV E ANGLIA,SCH BIOL SCI,NORWICH NR4 7TJ,NORFOLK,ENGLAND
关键词
nitrification; hydroxylamine-cytochrome c oxidoreductase; ammonia monooxygenase; denitrification; Pseudomonas sp;
D O I
10.1007/BF01682991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Hydroxylamine oxidation was measured in four recently isolated heterotrophic nitrate-reducing bacteria belonging to the genera Pseudomonas, Moraxella, Arthrobacter and Aeromonas. A hydroxylamine-cytochrome c oxidoreductase activity was detected in periplasmic fractions of the Pseudomonas and Aeromonas spp. and in total soluble fractions of the Arthrobacter sp. A monomeric 19-kDa non-haem iron hydroxylamine-cytochrome c oxidoreductase was purified from the Pseudomonas species and shown to be similar to hydroxylamine-cytochrome c oxidoreductase of Paracoccus denitrificans.
引用
收藏
页码:421 / 424
页数:4
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