A simple semi-quantitative approach studying the in vivo degradation of regenerated silk fibroin scaffolds with different pore sizes

被引：23
作者：
Guo, Yongwei [1 ]
Chen, Zhongchun [1 ]
Wen, Jianchuan [2 ,3 ]
Jia, Minghui [1 ]
Shao, Zhengzhong [2 ,3 ]
Zhao, Xia [1 ]
机构：
[1] Fudan Univ, Huashan Hosp, Dept Otorhinolaryngol Head & Neck Surg, 12 Wulumuqi Middle Rd, Shanghai 200040, Peoples R China
[2] Fudan Univ, Dept Macromol Sci, Shanghai 200433, Peoples R China
[3] Fudan Univ, Adv Mat Lab, Shanghai 200433, Peoples R China
来源：
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2017年 / 79卷
基金：
中国国家自然科学基金;
关键词：
In vivo degradation; Silk fibroin scaffold; Fibroblast; Inflammation; Pore size; MURINE MODEL; VITRO; FABRICATION; RESPONSES; DEFECT; FILMS;
D O I：
10.1016/j.msec.2017.05.008
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
The biocompatibility and in vivo degradation rate of biomaterials represent critical control points in the longterm success of scaffolds for tissue restoration. In this study, new three-dimensional (3D) regenerated silk fibroin scaffolds (RSFs) were prepared by the freezing-defrosting procedure, and then were implanted beneath the dorsal skin of rats. This study aims to develop a kinetic semi-quantitative approach to assess in vivo degradation rate and biocompatibility of this kind of RSFs with different pore sizes for the first time, and to evaluate the relationship between the biodegradation and tissue responses by measuring the thickness of residual scaffolds, fibrous capsules and infiltrated tissues through integrated techniques of histology, optical imaging and image analysis. Our results showed that scaffolds with both pore sizes (7435 +/- 10.84 mu m and 139.23 +/- 44.93 mu m, respectively) were well tolerated by host animals and pore size was found to be the rate limiting factor to the biodegradation in the subcutaneous implantation model. In addition, the biodegradation of RSFs was inflammation-mediated to a certain degree and fibroblasts may play a critical role in this process. Overall, such semi-quantitative approach was demonstrated to be a simple and effective method to assess the in vivo degradation rate, and the prepared RSFs were presented to have promising potential in tissue engineering applications. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：161 / 167
页数：7
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