Calorimetry of enzyme-catalyzed reactions

被引：63

作者：

Bianconi, M. Lucia ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Programa Biol Estrutural, Inst Bioquim Med, BR-21941 Rio De Janeiro, Brazil

来源：

BIOPHYSICAL CHEMISTRY | 2007年 / 126卷 / 1-3期

关键词：

calorimetric enzyme assays; thermodynamic of enzyme reactions;

D O I：

10.1016/j.bpc.2006.05.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This mini-review shows the valuable contributions of Professor Julian Sturtevant to the current applications of calorimetry to the study of enzyme-catalyzed reactions. The more recent applications of calorimetric techniques such as isothermal titration calorimetry and flow calorimetry to the study of enzyme kinetics, as well as the advantages on using calorimetric techniques in the determination of kinetic parameters of enzymes, is also discussed here. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：59 / 64

页数：6

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[2]

Bianconi M. L., 2004, BIOCALORIMETRY, V2, P175, DOI [10.1002/0470011122.ch9, DOI 10.1002/0470011122.CH9]

[3] Calorimetric determination of thermodynamic parameters of reaction reveals different enthalpic compensations of the yeast hexokinase isozymes [J].