A piezoelectric quartz crystal sensor for the determination of coagulation time in plasma and whole blood

被引：45

作者：

Cheng, TJ

Chang, HC ^{[1
]}

Lin, TM

机构：

[1] Natl Cheng Kung Univ, Inst Biomed Engn, Tainan 70101, Taiwan

[2] Natl Cheng Kung Univ, Dept Med Technol, Tainan 70101, Taiwan

来源：

BIOSENSORS & BIOELECTRONICS | 1998年 / 13卷 / 02期

关键词：

piezoelectric quartz crystal; blood coagulation; prothrombin time; clotting time; recalcification time;

D O I：

10.1016/S0956-5663(97)00111-5

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A Piezoelectric Quartz Crystal (PQC) system possessing an adequate sensitivity to a slight viscosity change is employed to monitor the blood coagulation. In the case of an anticoagulated plasma specimen, prothrombin time (PT) was determined by the PQC sensor on the basis of the introduction of tissue thromboplastin (TF) and calcium ion. Discussing the PQC data statistically, we found that at 90% of the total frequency shift (Delta f) the best linear relationship in a double-logarithmic plot of PT versus TF concentration in the range of 5.466 similar to 22.311 unit/ml is shown. The PQC result was compared with commercial optical coagulometry and showed a good linear correlation (ca. 0.98). The clotting and recalcification of whole blood were also studied by the PQC system. The testing time for one measurement could be completed within 20 min. and had a good agreement with that obtained conventionally. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：147 / 156

页数：10

共 12 条

[1] LECTIN COATED PIEZOELECTRIC CRYSTAL BIOSENSORS [J].