Imaging cell surface glycans with bioorthogonal chemical reporters

被引：166

作者：

Chang, Pamela V.

Prescher, Jennifer A.

Hangauer, Matthew J.

Bertozzi, Carolyn R. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 27期

关键词：

D O I：

10.1021/ja070238o

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The visualization of biomolecules as they function in living systems is important for understanding complex biological processes. Here, we applied the bioorthogonal chemical reporter technique to image cell surface glycans using multiple metabolic labels. We introduced two different chemical reporters into sialic acid and N-acetylgalactosamine (GalNAc) residues and then simultaneously imaged their associated cell surface glycans with fluorescent probes.

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页码：8400 / +

页数：3

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