Imaging cell surface glycans with bioorthogonal chemical reporters

被引：164
作者：
Chang, Pamela V.
Prescher, Jennifer A.
Hangauer, Matthew J.
Bertozzi, Carolyn R. [1 ]
机构：
[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[3] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 27期
关键词：
D O I：
10.1021/ja070238o
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The visualization of biomolecules as they function in living systems is important for understanding complex biological processes. Here, we applied the bioorthogonal chemical reporter technique to image cell surface glycans using multiple metabolic labels. We introduced two different chemical reporters into sialic acid and N-acetylgalactosamine (GalNAc) residues and then simultaneously imaged their associated cell surface glycans with fluorescent probes.
引用
收藏
页码：8400 / +
页数：3
相关论文
共 19 条
  • [1] A strain-promoted [3+2] azide-alkyne cycloaddition for covalent modification of blomolecules in living systems
    Agard, NJ
    Prescher, JA
    Bertozzi, CR
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (46) : 15046 - 15047
  • [2] Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase
    Chen, I
    Howarth, M
    Lin, WY
    Ting, AY
    [J]. NATURE METHODS, 2005, 2 (02) : 99 - 104
  • [3] A metabolic labeling approach toward proteomic analysis of mucin-type O-linked glycosylation
    Hang, HC
    Yu, C
    Kato, DL
    Bertozzi, CR
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (25) : 14846 - 14851
  • [4] HUANG KM, 1993, J BIOL CHEM, V268, P9302
  • [5] Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation
    Kiick, KL
    Saxon, E
    Tirrell, DA
    Bertozzi, CR
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (01) : 19 - 24
  • [6] A fluorogenic dye activated by the Staudinger ligation
    Lemieux, GA
    de Graffenried, CL
    Bertozzi, CR
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (16) : 4708 - 4709
  • [7] Engineering chemical reactivity on cell surfaces through oligosaccharide biosynthesis
    Mahal, LK
    Yarema, KJ
    Bertozzi, CR
    [J]. SCIENCE, 1997, 276 (5315) : 1125 - 1128
  • [8] Molecular imaging in living subjects: seeing fundamental biological processes in a new light
    Massoud, TF
    Gambhir, SS
    [J]. GENES & DEVELOPMENT, 2003, 17 (05) : 545 - 580
  • [9] Practical synthetic route to functionalized rhodamine dyes
    Nguyen, T
    Francis, MB
    [J]. ORGANIC LETTERS, 2003, 5 (18) : 3245 - 3248
  • [10] Chemistry in living systems
    Prescher, JA
    Bertozzi, CR
    [J]. NATURE CHEMICAL BIOLOGY, 2005, 1 (01) : 13 - 21
12