Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation

被引:22
作者
Hollmann, Birte [1 ,2 ]
Perkins, Mark [1 ,2 ]
Chauhan, Veeren M. [2 ]
Aylott, Jonathan W. [2 ]
Hardie, Kim R. [1 ]
机构
[1] Univ Nottingham, Sch Life Sci, Biodiscovery Inst, Univ Pk, Nottingham, England
[2] Univ Nottingham, Sch Pharm, Adv Mat & Healthcare Technol, Nottingham, England
基金
英国生物技术与生命科学研究理事会;
关键词
PSEUDOMONAS-AERUGINOSA; IN-VITRO; STREPTOCOCCUS-MUTANS; OPTICAL NANOSENSORS; XYLITOL INHIBITION; INTRACELLULAR PH; EXTRACELLULAR PH; ACID PRODUCTION; DIFFUSION; VIVO;
D O I
10.1038/s41522-021-00221-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Understanding the dynamic environmental microniches of biofilms will permit us to detect, manage and exploit these communities. The components and architecture of biofilms have been interrogated in depth; however, little is known about the environmental microniches present. This is primarily because of the absence of tools with the required measurement sensitivity and resolution to detect these changes. We describe the application of ratiometric fluorescent pH-sensitive nanosensors, as a tool, to observe physiological pH changes in biofilms in real time. Nanosensors comprised two pH-sensitive fluorophores covalently encapsulated with a reference pH-insensitive fluorophore in an inert polyacrylamide nanoparticle matrix. The nanosensors were used to analyse the real-time three-dimensional pH variation for two model biofilm formers: (i) opportunistic pathogen Pseudomonas aeruginosa and (ii) oral pathogen Streptococcus mutans. The detection of sugar metabolism in real time by nanosensors provides a potential application to identify therapeutic solutions to improve oral health.
引用
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页数:13
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