Synthesis, characterization and DNA binding studies of two Ru(II) complexes containing guanidinium ligands

被引：11

作者：

Chen, Wen-Xiu ^{[1
]}

Song, Xing-Dong ^{[1
]}

Chen, Jia-Xi ^{[1
]}

Zhao, Xuan-Hao ^{[1
]}

Xing, Jie-Hua ^{[1
]}

Ren, Jun-Rui ^{[1
]}

Wu, Tie ^{[1
]}

Sun, Jing ^{[1
,2
]}

机构：

[1] Guangdong Med Univ, Sch Pharm, Dongguan 523808, Peoples R China

[2] Guangdong Med Univ, Guangdong Key Lab Res & Dev Nat Drugs, Zhanjiang 524023, Peoples R China

来源：

POLYHEDRON | 2016年 / 110卷

基金：

中国国家自然科学基金;

关键词：

Synthesis; Ru(II) complex; G-quadruplex DNA; Guanidinium; H-telo; TELOMERIC G-QUADRUPLEX; POTASSIUM SOLUTION; RUTHENIUM(II) COMPLEXES; METAL-COMPLEXES; STABILIZATION; CANCER; 2,2-BIPYRIDINE; PHOTOCLEAVAGE; AFFINITY; BIOLOGY;

D O I：

10.1016/j.poly.2016.02.038

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Two new Ru(II) complexes containing guanidinium groups have been synthesized, characterized and analyzed according to their interactions with different G-quadruplexes and duplex DNA. A FRET assay and a competitive FRET assay showed that both complexes promote the formation and stabilization of the human telomeric (h-telo) G-quadruplex and exhibit higher selectivity compared to promoters (such as c-myc, c-kit and bcl2) or duplex DNA. After binding to G-quadruplex, the two complexes have shown different DNA affinity and fluorescence enhancement. CD analyses further indicate that the two complexes display the ability to induce and stabilize the formation of antiparallel G-quadruplex structures in K+, Na+ or ion-free buffers. The binding stoichiometry with h-telo was of the order of three ruthenium complexes per quadruplex. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：274 / 281

页数：8

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