Suppression of Carnosine on Adhesion and Extravasation of Human Colorectal Cancer Cells

被引：9

作者：

Wu, Chih-Chung ^{[1
]}

Lai, Po-Yu ^{[2
]}

Hsieh, Shuchen ^{[3
]}

Cheng, Ching-Ching ^{[4
]}

Hsieh, Shu-Ling ^{[2
]}

机构：

[1] Providence Univ, Dept Food & Nutr, Taichung, Taiwan

[2] Natl Kaohsiung Univ Sci & Technol, Dept Seafood Sci, 142 Haijhuan Rd, Kaohsiung 81157, Taiwan

[3] Natl Sun Yat Sen Univ, Dept Chem, Kaohsiung, Taiwan

[4] Natl ChiaTung Agr Vocat Senior High Sch, Dept Food Proc, Pingtung, Taiwan

来源：

ANTICANCER RESEARCH | 2019年 / 39卷 / 11期

关键词：

Carnosine; cell adhesion; extravasation; permeability; NF-kappa B signaling pathway; NF-KAPPA-B; TUMOR-CELLS; ENDOTHELIAL-CELLS; SIGNALING PATHWAY; E-SELECTIN; PERMEABILITY; ACTIVATION; INVASION; ICAM-1; INFLAMMATION;

D O I：

10.21873/anticanres.13821

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Aim: To investigate the effect of carnosine, an active compound of dietary beef, fish and chicken, on the regulation of cell adhesion and extravasation during metastasis. Materials and Methods: Cell adhesion and extravasation abilities, and related regulating molecular mechanisms were analyzed in human colorectal cancer cells (HCT-116) and human umbilical vein cells (EA.hy926). Results: Carnosine reduced the ability of HCT-116 cells to adhere to EA.hy926 cells. The expression levels of integrin-beta 1 in HCT-116 cells, as well as of intercellular adhesion molecule-1 and E-selectin in EA.hy926 cells, were reduced after carnosine treatment. After EA.hy926 cells were treated with carnosine, phosphorylation of vascular endothelia-cadherin (VE-cadherin), protein levels of Ras homologous (RHO) and RHO-associated coiled-coil containing protein kinase, and levels of reactive oxygen species were reduced. After treating EA.hy926 cells with carnosine, phosphorylation of inhibitor of kappa B (I kappa B) and DNA binding activity of nuclear factor-kappa B (NF-kappa B) were reduced. Conclusion: Carnosine inhibits metastatic cell adhesion and extravasation by suppressing NF-kappa B signaling activation.

引用

页码：6135 / 6144

页数：10

共 57 条

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