Autofocusing Algorithm for Pixel-Super-Resolved Lensfree On-Chip Microscopy

被引:11
作者
Wu, Yumin [1 ,2 ,3 ,4 ]
Lu, Linpeng [1 ,2 ,3 ,4 ]
Zhang, Jialin [1 ,2 ,3 ,4 ]
Li, Zhuoshi [1 ,2 ,3 ,4 ]
Zuo, Chao [1 ,2 ,3 ,4 ]
机构
[1] Nanjing Univ Sci & Technol, Sch Elect & Opt Engn, Nanjing, Peoples R China
[2] Nanjing Univ Sci & Technol, Jiangsu Key Lab Spectral Imaging & Intelligent Se, Nanjing, Peoples R China
[3] Nanjing Univ Sci & Technol, Smart Computat Imaging Lab, Nanjing, Peoples R China
[4] Nanjing Univ Sci & Technol, Inst Smart Computat Imaging, Nanjing, Peoples R China
基金
中国国家自然科学基金;
关键词
lensfree on-chip imaging; autofocusing; super-resolution; phase retrieval; computational imaging;
D O I
10.3389/fphy.2021.651316
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
In recent years, lensfree on-chip microscopy has developed into a promising and powerful computational optical microscopy technique that allows for wide-field, high-throughput microscopic imaging without using any lenses. However, due to the limited pixel size of the state-of-the-art image sensors, lens-free on-chip microscopy generally suffers from low imaging resolution, which is far from enough to meet the current demand for high-resolution microscopy. Many pixel super-resolution techniques have been developed to solve or at least partially solve this problem by acquiring a series of low-resolution holograms with multiple lateral sub-pixel shifting or axial distances. However, the prerequisite of these pixel super-resolution techniques is that the propagation distance of each low-resolution hologram can be obtained precisely, which faces two major challenges. On the one hand, the captured hologram is inherent pixelated and of low resolution, making it difficult to determine the focal plane by evaluating the image sharpness accurately. On the other hand, the twin-image is superimposed on the backpropagated raw hologram, further exacerbating the difficulties in accurate focal plane determination. In this study, we proposed a high-precision autofocusing algorithm for multi-height pixel-super-resolved lensfree on-chip microscopy. Our approach consists of two major steps: individual preliminary estimation and global precise estimation. First, an improved critical function that combines differential critical function and frequency domain critical function is proposed to obtain the preliminary focus distances of different holograms. Then, the precise focus distances can be determined by further evaluating the global offset of the averaged, low-noise reconstruction from all backpropagated holograms with preliminary focus distances. Simulations and experimental results verified the validity and effectiveness of the proposed algorithm.
引用
收藏
页数:12
相关论文
共 34 条
  • [1] Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array
    Bishara, Waheb
    Sikora, Uzair
    Mudanyali, Onur
    Su, Ting-Wei
    Yaglidere, Oguzhan
    Luckhart, Shirley
    Ozcan, Aydogan
    [J]. LAB ON A CHIP, 2011, 11 (07) : 1276 - 1279
  • [2] Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution
    Bishara, Waheb
    Su, Ting-Wei
    Coskun, Ahmet F.
    Ozcan, Aydogan
    [J]. OPTICS EXPRESS, 2010, 18 (11): : 11181 - 11191
  • [3] Albumin testing in urine using a smart-phone
    Coskun, Ahmet F.
    Nagi, Richie
    Sadeghi, Kayvon
    Phillips, Stephen
    Ozcan, Aydogan
    [J]. LAB ON A CHIP, 2013, 13 (21) : 4231 - 4238
  • [4] Focus plane detection criteria in digital holography microscopy by amplitude analysis
    Dubois, Frank
    Schockaert, Cedric
    Callens, Natacha
    Yourassowsky, Catherine
    [J]. OPTICS EXPRESS, 2006, 14 (13): : 5895 - 5908
  • [5] Autofocusing based on wavelength dependence of diffraction in two-wavelength digital holographic microscopy
    Gao, Peng
    Yao, Baoli
    Rupp, Romano
    Min, Junwei
    Guo, Rongli
    Ma, Baiheng
    Zheng, Juanjuan
    Lei, Ming
    Yan, Shaohui
    Dan, Dan
    Ye, Tong
    [J]. OPTICS LETTERS, 2012, 37 (07) : 1172 - 1174
  • [6] Immersion digital in-line holographic microscopy
    Garcia-Sucerquia, J
    Xu, WB
    Jericho, MH
    Kreuzer, HJ
    [J]. OPTICS LETTERS, 2006, 31 (09) : 1211 - 1213
  • [7] THE USE OF SELF-ENTROPY AS A FOCUS MEASURE IN DIGITAL HOLOGRAPHY
    GILLESPIE, J
    KING, RA
    [J]. PATTERN RECOGNITION LETTERS, 1989, 9 (01) : 19 - 25
  • [8] Subjective assessment of HDTV with superresolution function
    Gohshi, Seiichi
    Hiroi, Takayuki
    Echizen, Isao
    [J]. EURASIP JOURNAL ON IMAGE AND VIDEO PROCESSING, 2014,
  • [9] Wide-field computational imaging of pathology slides using lens-free on-chip microscopy
    Greenbaum, Alon
    Zhang, Yibo
    Feizi, Alborz
    Chung, Ping-Luen
    Luo, Wei
    Kandukuri, Shivani R.
    Ozcan, Aydogan
    [J]. SCIENCE TRANSLATIONAL MEDICINE, 2014, 6 (267)
  • [10] Joint MAP registration and high-resolution image estimation using a sequence of undersampled images
    Hardie, RC
    Barnard, KJ
    Armstrong, EE
    [J]. IEEE TRANSACTIONS ON IMAGE PROCESSING, 1997, 6 (12) : 1621 - 1633