Hydrogen-bonding-induced colorimetric detection of melamine by nonaggregation-based Au-NPs as a probe

被引:93
作者
Cao, Qian [1 ]
Zhao, Hong [1 ]
He, Yujian [1 ,2 ]
Li, Xiangjun [1 ]
Zeng, Lixi [1 ]
Ding, Nan [1 ]
Wang, Jian [1 ]
Yang, Jie [1 ]
Wang, Guangwei [3 ]
机构
[1] Chinese Acad Sci, Grad Univ, Coll Chem & Chem Engn, Beijing 100049, Peoples R China
[2] Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
[3] Hunan Normal Univ, Coll Med, Changsha 410006, Hunan, Peoples R China
基金
中国国家自然科学基金;
关键词
3,5-Dihydroxybenzoic acid; Melamine; Gold nanoparticles; Hydrogen-bonding; TANDEM MASS-SPECTROMETRY; GOLD NANOPARTICLES; CYANURIC ACID; AQUEOUS-SOLUTION; LIQUID-CHROMATOGRAPHY; INFANT FORMULA; COLOR-CHANGE; MILK; SPECTROSCOPY; AMMELIDE;
D O I
10.1016/j.bios.2010.04.046
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A colorimetric, label-free, and nonaggregation-based gold nanoparticles probe has been developed for the detection of melamine. Gold nanoparticles were generated using 3,5-dihydroxybenzoic acid as a reducer without adding gold nanoparticle seeds at room temperature. Upon the addition of melamine, the reducer 3,5-dihydroxybenzoic acid can interact with melamine through strong hydrogen-bonding interaction. Consequently, the formation of gold nanoparticles was interrupted by melamine since there was not enough reducer for the reduction of Au3+ ion. And the color change from purple to yellowgreen with increasing melamine concentration was observed. The plasmon absorbance of the formed Au-NPs allows the quantitative detection of melamine. A sensitive linear correlation existed between the absorbance and the logarithm of melamine concentration ranging from 1 x 10(-9) M to 1 x 10(-5) M with a linear coefficiency of 0.993. The system has a high selectivity to melamine with a low detection limit of 8 x 10(-10) M. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:2680 / 2685
页数:6
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