Circulating exosomes may identify biomarkers for cows at risk for metabolic dysfunction

被引：15

作者：

Almughlliq, Fatema B. ^{[1
]}

Koh, Yong Q. ^{[1
]}

Peiris, Hassendrini N. ^{[2
]}

Vaswani, Kanchan ^{[2
]}

Holland, Olivia ^{[2
]}

Meier, Susanne ^{[3
]}

Roche, John R. ^{[3
,4
]}

Burke, Chris R. ^{[3
]}

Crookenden, Mallory A. ^{[3
]}

Arachchige, Buddhika J. ^{[1
]}

Reed, Sarah ^{[1
]}

Mitchell, Murray D. ^{[2
]}

机构：

[1] Univ Queensland, Fac Med, Ctr Clin Res, Brisbane, Qld 4029, Australia

[2] Queensland Univ Technol, Fac Hlth, Ctr Childrens Hlth Res, Inst Hlth & Biomed Innovat, Brisbane, Qld 4101, Australia

[3] DairyNZ Ltd, Private Bag 3221, Hamilton 3240, New Zealand

[4] Minist Primary Ind Manatu Ahu Matua, Pastoral House, Wellington 6140, New Zealand

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

基金：

澳大利亚研究理事会;

关键词：

DAIRY-COWS; STROMAL CELLS; PROSTAGLANDIN E-2; EXPRESSION; PROTEIN; CYCLOOXYGENASES; COMMUNICATION; INFLAMMATION; PATHWAY; INHIBIN;

D O I：

10.1038/s41598-019-50244-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Disease susceptibility of dairy cows is greatest during the transition from pregnancy to lactation. Circulating exosomes may provide biomarkers to detect at-risk cows to enhance health and productivity. From 490 cows, animals at high-(n = 20) or low-risk (n = 20) of transition-related diseases were identified using plasma non-esterified fatty acid and beta-hydroxybutyrate concentrations and liver triacylglyceride concentrations during the two weeks post-calving. We isolated circulating exosomes from plasma of dairy cows at low-risk (LR-EXO) and high-risk (HR-EXO), and analyzed their proteome profiles to determine markers for metabolic dysfunction. We evaluated the effects of these exosomes on eicosanoid pathway expression by bovine endometrial stromal (bCSC) and epithelial (bEEL) cells. HR-EXO had significantly lower yield of circulating exosomes compared with LR-EXO, and unique proteins were identified in HR-EXO and LR-EXO. Exposure to LR-EXO or HR-EXO differentially regulated eicosanoid gene expression and production in bCSC and bEEL cells. In bCSC, LR-EXO exposure increased PGE(2) and PGD(2) production, whereas HR-EXO exposure increased PTGS2 gene expression. In bEEL, HR-EXO exposure caused a decrease in PGE(2), PGF(2 alpha), PGD(2), PGFM and TXB2 production. The unique presence of serpin A3-7, coiled-coil domain containing 88A and inhibin/ activin beta A chain in HREXO, indicates potential biomarkers for cows at-risk for metabolic diseases. Our results are in line with the health status of the cow indicating a potential diagnostic role for exosomes in enhancing cows' health and fertility.

引用

页数：12

共 64 条

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