High-porosity activated carbon as a possible matrix for native DNA and dextran-sulfate immobilization

被引:3
作者
Snezkova, EA
Muller, D
Bardakhivskaya, KI
Mikhalovsky, SV
Nikolaev, VG
机构
[1] Natl Acad Sci Ukraine, RE Kavetsky Inst Expt Pathol Oncol & Radiobiol, UA-03022 Kiev, Ukraine
[2] Ecole Natl Super Chim Paris, F-75231 Paris 05, France
[3] Univ Brighton, Sch Pharm & Biomol Sci, Brighton, E Sussex, England
来源
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2004年 / 32卷 / 04期
关键词
carbonic adsorbents; fractality; dextran-sulfate; DNA; cytokines;
D O I
10.1081/BIO-200039612
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In this study, specific and nonspecific activity of calf thymus DNA and Dextran-Sulfate (DS)-containing synthetic carbonic adsorbents (0.7-7 mg of each ligands per 1 cm(3) of activated carbonic beads, 0.3-0.6 mm diameter, bulk density gamma=0.1-0.2 g/cm(3)) have been compared in stir-bath and micro-column in vitro tests. DS coating as well as DNA coating does not demonstrate deep influence on the unspecific adsorptive activity of carbonic matrix toward creatinine, vitamin B-12, and unconjugated bilirubin. No essential difference has been found in the specific activity of DNA and DS containing adsorbents toward anti-ds- and anti-ss-DNA-anti bodies, as well as antibodies against DNA-protein complexes (anti-DNP-antibodies): in both cases the percentage of decrease of appropriate antibody concentration varied between 35 and 51% for single-pass microcolumn experiments with moderate enhancement of extraction efficacy (up to 60-75%) due to additional recirculation (2 h) or preliminary plasma dilution in 2-5 times. In the micro-column experiments with the proinflammatory cytokines DNA or DS-coating did not diminish TNF-alpha, IL-1beta, and IL-6 adsorption from 3% BSA solution, but even improves to some extent its removal compared with uncoated matrix.
引用
收藏
页码:529 / 537
页数:9
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