Anti-inflammatory effect of water extracts obtained from doenjang in LPS-stimulated RAW 264.7 cells

被引：9

作者：

Lee, Sang Hyun ^{[1
]}

Lee, Hyesook ^{[2
,3
]}

Kim, Jae Cherl ^{[1
]}

机构：

[1] Inje Univ, Dept Smart Foods & Drugs, Gimhae 621749, Gyeongnam, South Korea

[2] Dong Eui Univ, Coll Korean Med, Antiaging Res Ctr, Busan 47340, South Korea

[3] Dong Eui Univ, Coll Korean Med, Dept Biochem, Busan 47340, South Korea

来源：

FOOD SCIENCE AND TECHNOLOGY | 2019年 / 39卷 / 04期

关键词：

water extracts of doenjang (WED); fermented soybean paste; oxidative stress; inflammation; Lipopolysaccharide; NF-KAPPA-B; BIOACTIVE PEPTIDES; INHIBITORY PEPTIDE; OXIDATIVE STRESS; NITRIC-OXIDE; ACTIVATION; CYCLOOXYGENASE-2; MACROPHAGES; PROTEINS; LUNASIN;

D O I：

10.1590/fst.15918

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The effect of water extracts of doenjang (WED) on cyclooxygenase pathway and cytokine production in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells was investigated. WED was fractionated by ultrafiltration into five individual groups of molecular weights. All of the WED fractions significantly inhibited the production of prostaglandin E-2 (PGE(2)) and expression of cyclooxygenase-2 (COX-2) in LPS-stimulated RAW 264.7 macrophages. Furthermore, each of the WED fractions significantly decreased production of pro-inflammatory cytokine such as tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) (p<0.05). The fraction between 10 to 30 kDa of WED showed the highest inhibition of pro-inflammatory cytokine production, LPS-induced COX-2 expression and PEG(2) production. These results suggest that WED fractions may have the anti-inflammatory effect via the stimulus of COX-2 and TNF-alpha in LPS-stimulated inflammation in RAW 264.7 cells.

引用

页码：947 / 954

页数：8

共 32 条

[1]

Ahmad N, 2002, J LEUKOCYTE BIOL, V71, P1005

[2]

[Anonymous], 2012, Ph.D. thesis

[3] Dual acting anti-inflammatory drugs: A reappraisal [J].

Bertolini, A ;

Ottani, A ;

Sandrini, M .

PHARMACOLOGICAL RESEARCH, 2001, 44 (06) :437-450

[4]

BOREK C, 1991, FREE RADICAL RES COM, V12-3, P745

[5] Oxidative stress and nuclear factor-κB activation -: A reassessment of the evidence in the light of recent discoveries [J].

Bowie, A ;

O'Neill, LAJ .

BIOCHEMICAL PHARMACOLOGY, 2000, 59 (01) :13-23

[6] RGD-peptide lunasin inhibits Akt-mediated NF-?B activation in human macrophages through interaction with the αVβ3 integrin [J].

Cam, Anthony ;

de Mejia, Elvira Gonzalez .

MOLECULAR NUTRITION & FOOD RESEARCH, 2012, 56 (10) :1569-1581

[7] Production and processing of soybeans and nutrition and safety of isoflavone and other soy products for human health [J].

Choi, Myung-Sook ;

Rhee, Khee Choon .

JOURNAL OF MEDICINAL FOOD, 2006, 9 (01) :1-10

[8]

de Lumen BO, 2005, NUTR REV, V63, P16, DOI [10.1301/nr.2004.janr.16-21, 10.1111/j.1753-4887.2005.tb00106.x]

[9] Production and characterization of bioactive peptides from soy hydrolysate and soy-fermented food [J].

Gibbs, BF ;

Zougman, A ;

Masse, R ;

Mulligan, C .

FOOD RESEARCH INTERNATIONAL, 2004, 37 (02) :123-131

[10] Angiotensin I-converting enzyme inhibitory peptide derived from glycinin, the 11S globulin of soybean (Glycine max) [J].

Gouda, K. G. Mallikarjun ;

Gowda, Lalitha R. ;

Rao, A. G. Appu ;

Prakash, V. .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (13) :4568-4573

← 1 2 3 4 →