Strand Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2

被引：32

作者：

Hogan, Catherine A. ^{[1
,2
]}

Huang, ChunHong ^{[1
]}

Sahoo, Malaya K. ^{[1
]}

Wang, Hannah ^{[1
]}

Jiang, Becky ^{[2
]}

Sibai, Mamdouh ^{[2
]}

Holubar, Marisa ^{[1
]}

Mathew, Roshni ^{[1
]}

Zehnder, James ^{[1
]}

Pinsky, Benjamin A. ^{[1
,2
]}

机构：

[1] Stanford Univ, Sch Med, Stanford, CA 94305 USA

[2] Stanford Hlth Care, Stanford, CA USA

来源：

EMERGING INFECTIOUS DISEASES | 2021年 / 27卷 / 02期

关键词：

D O I：

10.3201/eid2702.204168

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about patient infectiousness.

引用

页码：632 / 635

页数：4

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