Exogenous trehalose protects photosystem II by promoting cyclic electron flow under heat and drought stresses in winter wheat

Drought and rising global temperatures are important factors that reduce wheat production. Trehalose protects the reaction centres and improves photosystem II (PSII) activity under diverse stress conditions. However, the underlying mechanism remains unknown. Cyclic electron flow (CEF) plays an important role in protecting PSII under environmental stresses. Our study focused on the effects of exogenous trehalose on the activity of PSII, D1 protein content, plastoquinone (PQ) pool and ATP synthase activity in wheat seedlings under heat and drought stresses to explore the relationship between trehalose and CEF. The results indicated that heat and drought stresses decreased maximum photochemical efficiency of PSII (F-v/F-m) and electron transport rate of PSII (EFR(II)), whereas the trehalose pretreatment improved photochemical efficiency and electron transport rate of PSII. The trehalose pretreatment stimulated CEF under heat and drought stresses. Furthermore, the proton gradient (Delta pH) across the thylakoid membrane and ATPase activity increased. The higher Delta pH and ATPase activity played a key role in protecting PSII under stresses. Trehalose pretreatment could reduce inhibition caused by heat and drought stresses on the PQ pool. Thus, our results indicated that photoinhibition in heat- and drought-stressed plants was alleviated by the trehalose pretreatment, which was mediated by CEF and the PQ pool.