Synthesis of methylketone containing nucleoside triphosphates for RNA labelling

被引:26
作者
Trévisiol, E
Defrancq, E
Lhomme, J
Laayoun, A
Cros, P
机构
[1] Univ Grenoble 1, Etud Dynam & Struct Select Lab, CNRS, UMR 5616, F-38041 Grenoble 9, France
[2] BioMerieux, Lab Sondes Nucl, F-69280 Marcy Etoile, France
关键词
fluorescence; labelling; ketone; nucleotides; phosphorylation;
D O I
10.1016/S0040-4020(00)00577-9
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
The three nucleoside triphosphates 1, 2 and 3 bearing a linker with a terminal methylketone group were prepared for incorporation into RNA fragments and post-labelling by the fluorescein derivative 4 containing an aminooxy group. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:6501 / 6510
页数:10
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