Temporin L-derived peptide as a regulator of the acute inflammatory response in zymosan-induced peritonitis

被引：20

作者：

Bellavita, Rosa ^{[1
]}

Raucci, Federica ^{[1
]}

Merlino, Francesco ^{[1
]}

Piccolo, Marialuisa ^{[1
]}

Ferraro, Maria Grazia ^{[1
]}

Irace, Carlo ^{[1
]}

Santamaria, Rita ^{[1
]}

Iqbal, Asif J. ^{[2
]}

Novellino, Ettore ^{[1
]}

Grieco, Paolo ^{[1
]}

Mascolo, Nicola ^{[1
]}

Maione, Francesco ^{[1
]}

机构：

[1] Univ Naples Federico II, Sch Med & Surg, Dept Pharm, Via Domen Montesano 49, I-80131 Naples, Italy

[2] Univ Birmingham, Coll Med & Dent Sci, Inst Cardiovasc Sci ICVS, Birmingham B15 2TT, W Midlands, England

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2020年 / 123卷

关键词：

Antimicrobial peptides; Inflammation; Monocytes; Temporin-L; Zymosan; ANTIMICROBIAL PEPTIDES; IN-VIVO; MODEL; PUBLICATION; GLYCINE;

D O I：

10.1016/j.biopha.2019.109788

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Antimicrobial peptides (AMPs) are an ancient group of defense molecules distributed in nature being found in mammals, birds, amphibians, insects, plants, and microorganisms. They display antimicrobial as well as immunomodulatory properties. The aim of this study was to investigate, for the first time, the anti-inflammatory activities of two synthetic temporin-L analogues (here named peptide 1 and 2) by an in vivo model of inflammation caused by intraperitoneal sub-lethal dose of zymosan. Our results show that peptide 1 and 2 exert anti-inflammatory activity in vivo in response to zymosan-induce peritonitis. Simultaneous administration of 10 mg/kg of both temporins, with a sub-lethal dose of zymosan (500 mg/kg), significantly rescued mice from the classical hallmarks of inflammation, including leukocyte infiltration and synthesis of inflammatory mediators including IL-6, TNF-alpha and MCP-1. More importantly, flow cytometry analysis highlighted a selective modulation of infiltrating inflammatory monocytes (defined as B220(-)/GR1(hi)-F480(hi)/CD115(+)) after peptide 2 treatment. Our results and presented models offer the possibility to test, in a preclinical setting, the potential of temporin analogues as anti-inflammatory agents.

引用

页数：7

共 37 条

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