NO-synthase gene and angiotensin receptor II (type 1) gene polymorphism and endothelium-related hemostasis in patients with coronary heart disease

Relationship between polymorphisms of NO-synthase (NOS3) and angiotensin receptor II (type 1) (AT2R1) genes and endothelial-related hemostasis was assessed in 93 patients with coronary heart disease (52 males and 41 females; mean age 61+/-1,3 years). Venous-occlusion test (VOT) was performed to study endothelial hemostasis. Protein C, von Willebrand factor, plasminogen activator inhibitor (PAI) plasma concentrations were measured for antithrombotic endothelial function estimation To identify alleles of NOS3 and AT2R1 polymorphic markers we performed polymerase chain reaction amplification We found an association between Glu allele of the NOS3 gene (Glu298Asp polymorphism) and protein C consumption in VOT (chi (2)=6,83, p=0,033). Significant differences in protein C dynamics in VOT between patients with bb genotype of NOS gene and patients with aa and ab genotypes occurred (3,3+/-2,23% and -1,5+/-1,43%, p=0,028). In patients with protein C consumption in VOT frequency of AA genotype AT2R1 was significantly higher than AC and CC genotypes.