Identity of blaCTX-M Carrying Plasmids in Sequential ESBL-E. coli Isolates from Patients with Recurrent Urinary Tract Infections

被引：9

作者：

Karami, Nahid ^{[1
,2
,3
]}

KK, Sriram ^{[4
]}

Yazdanshenas, Shora ^{[1
,2
,3
]}

Lin, Yii-Lih ^{[4
]}

Jaen-Luchoro, Daniel ^{[1
,2
]}

Ekedahl, Elina ^{[4
]}

Parameshwaran, Sanjana ^{[4
]}

Lindblom, Anna ^{[1
,2
,3
]}

Ahren, Christina ^{[1
,2
,5
]}

Westerlund, Fredrik ^{[4
]}

机构：

[1] Univ Gothenburg, Dept Infect Dis, Inst Biomed, Guldhedsgatan 10 A, S-41346 Gothenburg, Sweden

[2] Univ Gothenburg, Ctr Antibiot Resistance Res CARe, Guldhedsgatan 10 A, S-41346 Gothenburg, Sweden

[3] Sahlgrens Univ Hosp, Vastra Gotaland Reg, Dept Clin Microbiol, Guldhedsgatan 10A, S-41346 Gothenburg, Sweden

[4] Chalmers Univ Technol, Dept Biol & Biol Engn, Kemivagen 10, S-41296 Gothenburg, Sweden

[5] Vastra Gotaland Reg, Swedish Strateg Program Antimicrobial Resistance, S-40544 Gothenburg, Sweden

来源：

MICROORGANISMS | 2021年 / 9卷 / 06期

基金：

欧盟地平线“2020”;

关键词：

extended-spectrum beta-lactamase (ESBL); recurrent urinary tract infection; plasmids; horizontal transfer; E; coli; optical DNA mapping (ODM); ESCHERICHIA-COLI; RESISTANCE PLASMIDS; OUTBREAK; IDENTIFICATION; DIVERSITY; STRAINS; GENES; CLADE;

D O I：

10.3390/microorganisms9061138

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Plasmid-mediated multidrug resistance in E. coli is becoming increasingly prevalent. Considering this global threat to human health, it is important to understand how plasmid-mediated resistance spreads. From a cohort of 123 patients with recurrent urinary tract infections (RUTI) due to extended spectrum beta-lactamase (ESBL)-producing Escherichia coli (ESBL E. coli), only five events with a change of ESBL E. coli strain between RUTI episodes were identified. Their bla(CTX-M) encoding plasmids were compared within each pair of isolates using optical DNA mapping (ODM) and PCR-based replicon typing. Despite similar bla(CTX-M) genes and replicon types, ODM detected only one case with identical plasmids in the sequential ESBL E. coli strains, indicating that plasmid transfer could have occurred. For comparison, plasmids from seven patients with the same ESBL E. coli strain reoccurring in both episodes were analyzed. These plasmids (encoding bla(CTX-M-3), bla(CTX-M-14), and bla(CTX-M-15)) were unaltered for up to six months between recurrent infections. Thus, transmission of bla(CTX-M) plasmids appears to be a rare event during the course of RUTI. Despite the limited number (n = 23) of plasmids investigated, similar bla(CTX-M)-(15) plasmids in unrelated isolates from different patients were detected, suggesting that some successful plasmids could be associated with specific strains, or are more easily transmitted.

引用

页数：12