Bacillus amyloliquefaciens strain MBI600 induces salicylic acid dependent resistance in tomato plants against Tomato spotted wilt virus and Potato virus Y

被引：102
作者：
Beris, Despoina [1 ]
Theologidis, Ioannis [1 ]
Skandalis, Nicholas [1 ,2 ]
Vassilakos, Nikon [1 ]
机构：
[1] Fdn Res & Technol, Inst Mol Biol & Biotechnol, GR-71110 Iraklion, Greece
[2] Univ South Calif, Keck Sch Med, 2020 Zonal Ave,Off Campus, Los Angeles, CA USA
来源：
SCIENTIFIC REPORTS | 2018年 / 8卷
关键词：
SYSTEMIC ACQUIRED-RESISTANCE; ACIBENZOLAR-S-METHYL; DISEASE RESISTANCE; RNA-POLYMERASE; JASMONIC ACID; DEFENSE; GENE; INFECTION; BACTERIAL; BIOCONTROL;
D O I：
10.1038/s41598-018-28677-3
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Plant growth promoting rhizobacteria have been proposed as effective biocontrol agents against several fungal and bacterial plant pathogens. However, there is limited knowledge regarding their effect against viruses. In this study, Bacillus amyloliquefaciens strain MBI600 (MBI600), active ingredient of the biological fungicide Serifel (R) (BASF SE), was tested for its antiviral action in tomato plants. Drench, foliar or soil amendment applications of MBI600 reduced up to 80% the incidence of Tomato spotted wilt virus under two different sets of environmental conditions. In addition, drench application of MBI600 delayed Potato virus Y systemic accumulation. Transcriptional analysis of a range of genes associated with salicylic acid (SA)-or jasmonic acid - related defense, priming or basal defense against viruses, revealed the induction of the SA signaling pathway in tomato after MBI600 treatment, and discrete gene expression patterns in plant response to TSWV and PVY infection.
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页数：11
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共 54 条
[1]   Antagonistic Plant Defense System Regulated by Phytohormones Assists Interactions Among Vector Insect, Thrips and a Tospovirus [J].
Abe, Hiroshi ;
Tomitaka, Yasuhiro ;
Shimoda, Takeshi ;
Seo, Shigemi ;
Sakurai, Tamito ;
Kugimiya, Soichi ;
Tsuda, Shinya ;
Kobayashi, Masatomo .
PLANT AND CELL PHYSIOLOGY, 2012, 53 (01) :204-212
[2]   Salicylic acid-mediated and RNA-silencing defense mechanisms cooperate in the restriction of systemic spread of plum pox virus in tobacco [J].
Alamillo, Josefa M. ;
Saenz, Pilar ;
Garcia, Juan Antonio .
PLANT JOURNAL, 2006, 48 (02) :217-227
[3]   Roles of plant hormones in the regulation of host-virus interactions [J].
Alazem, Mazen ;
Lin, Na-Sheng .
MOLECULAR PLANT PATHOLOGY, 2015, 16 (05) :529-540
[4]   Salicylic acid is an indispensable component of the Ny-1 resistance-gene-mediated response against Potato virus Y infection in potato [J].
Baebler, S. ;
Witek, K. ;
Petek, M. ;
Stare, K. ;
Tusek-Znidaric, M. ;
Pompe-Novak, M. ;
Renaut, J. ;
Szajko, K. ;
Strzelczyk-Zyta, D. ;
Marczewski, W. ;
Morgiewicz, K. ;
Gruden, K. ;
Hennig, J. .
JOURNAL OF EXPERIMENTAL BOTANY, 2014, 65 (04) :1095-1109
[5]   Dynamics of Responses in Compatible Potato - Potato virus Y Interaction Are Modulated by Salicylic Acid [J].
Baebler, Spela ;
Stare, Katja ;
Kovac, Maja ;
Blejec, Andrej ;
Prezelj, Nina ;
Stare, Tjasa ;
Kogovsek, Polona ;
Pompe-Novak, Marusa ;
Rosahl, Sabine ;
Ravnikar, Maja ;
Gruden, Kristina .
PLOS ONE, 2011, 6 (12)
[6]   Mitogen-Activated Protein Kinases 3 and 6 Are Required for Full Priming of Stress Responses in Arabidopsis thaliana [J].
Beckers, Gerold J. M. ;
Jaskiewicz, Michal ;
Liu, Yidong ;
Underwood, William R. ;
He, Sheng Yang ;
Zhang, Shuqun ;
Conrath, Uwe .
PLANT CELL, 2009, 21 (03) :944-953
[7]   Involvement of lipopeptide antibiotics and chitinase genes and induction of host defense in suppression of Fusarium wilt by endophytic Bacillus spp. in tomato [J].
Ben Abdallah, Rania Aydi ;
Stedel, Catalina ;
Garagounis, Constantine ;
Nefzi, Ahlem ;
Jabnoun-Khiareddine, Hayfa ;
Papadopoulou, Kalliope K. ;
Daami-Remadi, Mejda .
CROP PROTECTION, 2017, 99 :45-58
[8]   Plant growth-promoting rhizobacteria (PGPR): Their potential as antagonists and biocontrol agents [J].
Beneduzi, Anelise ;
Ambrosini, Adriana ;
Passaglia, Luciane M. P. .
GENETICS AND MOLECULAR BIOLOGY, 2012, 35 (04) :1044-1051
[9]   Systemic acquired tolerance to virulent bacterial pathogens in tomato [J].
Block, A ;
Schmelz, E ;
O'Donnell, PJ ;
Jones, JB ;
Klee, HJ .
PLANT PHYSIOLOGY, 2005, 138 (03) :1481-1490
[10]   Biocontrol of tomato wilt disease by Bacillus subtilis isolates from natural environments depends on conserved genes mediating biofilm formation [J].
Chen, Yun ;
Yan, Fang ;
Chai, Yunrong ;
Liu, Hongxia ;
Kolter, Roberto ;
Losick, Richard ;
Guo, Jian-hua .
ENVIRONMENTAL MICROBIOLOGY, 2013, 15 (03) :848-864
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