Localization of disulfide bonds in α1-acid glycoprotein and their effect on the ability of the protein to interact with ethidium bromide

被引：0
|
作者：
Trubetskaya, OE [1 ]
Reznikova, OI
Afanas'eva, GV
Reznikov, MY
Serebryakova, MV
机构：
[1] Russian Acad Sci, Pushchino Branch, Shemyakin Ovchinnikov Inst Bioorgan Chem, Pushchino 142290, Moscow Oblast, Russia
[2] Russian Acad Med Sci, Mental Hlth Res Ctr, Moscow 109801, Russia
来源：
BIOORGANICHESKAYA KHIMIYA | 2000年 / 26卷 / 06期
关键词：
alpha 1-acid glycoprotein; orosomucoid; acute phase blood serum proteins;
D O I：
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中图分类号：
学科分类号：
摘要：
alpha 1-Acid glycoprotein (orosomucoid) was purified from the human and murine blood sera using phenol deproteinization. As opposed to the murine protein, the human orosomucoid bound the fluorescent dye ethidium bromide but lost this ability after treatment with beta-mercaptoethanol, which breaks disulfide bonds. Disulfide bonds between the Cys(23) and Cys(165) residues of the human orosomucoid and between the Cys(91) and Cys(184) residues of the murine orosomucoid were identified.
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页码：417 / 422
页数：6
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