Study on the bioavailability of stevioside-encapsulized lutein and its mechanism

被引：30

作者：

Dai, Zhuqing ^{[1
]}

Song, Jiangfeng ^{[1
]}

Chen, Ye ^{[1
]}

Feng, Lei ^{[1
]}

Xu, Yayuan ^{[1
]}

Li, Dajing ^{[1
]}

Wu, Caie ^{[2
]}

Zhang, Zhongyuan ^{[1
]}

Liu, Jun ^{[3
]}

机构：

[1] Jiangsu Acad Agr Sci, Inst Agroprod Proc, Nanjing 210014, Peoples R China

[2] Nanjing Forestry Univ, Coll Light Ind & Food Engn, Nanjing 210037, Peoples R China

[3] Yangzhou Univ, Coll Food Sci & Engn, Yangzhou 225127, Jiangsu, Peoples R China

来源：

FOOD CHEMISTRY | 2021年 / 354卷

基金：

中国国家自然科学基金;

关键词：

Lutein; Stevioside; Nanoparticle; Structural characteristics; Bioavailability; Mechanism; IN-VITRO; SR-BI; DELIVERY-SYSTEMS; CELLULAR UPTAKE; ABSORPTION; BIOACCESSIBILITY; NANOPARTICLES; TRANSPORT; HEALTH; ANTIOXIDANTS;

D O I：

10.1016/j.foodchem.2021.129528

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study aims to develop novel lutein nanoparticles encapsulized by stevioside (LUT-STE, 165 ? 2 nm average particles size) and systematically evaluate its bioavailability. Multiple spectroscopy and NMR analyses showed lutein and stevioside could interact through hydrogen bonds, C?H?n interaction and van der Waals forces. Molecular docking simulation showed lutein was well distributed in the hydrophobic cavity of stevioside. Analyzed by Caco-2 cellular models, the transported amount of LUT-STE was 2.39 times that of lutein in 120 min with a Papp (B ? A)/Papp (A ? B) value of 0.63 ? 0.04. Nystatin and dynasore significantly reduced the cellular uptake of LUT-STE by 41.3% and 57.7%, respectively. Compared with free lutein, LUT-STE increased the Cmax in mice plasma by 5.01-fold and promoted the accumulation in multiple organs. LUT-STE promoted the protein expressions of CD36, NPC1L1 and PPAR? in both cell and animal models. In conclusion, stevioside entrapment significantly promote the bioavailability of lutein through multiple transmembrane pathways.

引用

页数：11

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