Complete Genome Sequence of Phytopathogenic Pectobacterium atrosepticum Lytic Bacteriophage Peat2

被引:1
作者
Kalischuk, Melanie [1 ]
Hachey, John [1 ]
Thomas, Dallas [1 ]
Johnson, Dan [2 ]
Kawchuk, Lawrence [1 ]
机构
[1] Agr & Agri Food Canada, Lethbridge, AB, Canada
[2] Univ Lethbridge, Dept Geog, Lethbridge, AB, Canada
关键词
Blackleg; Pectobacterium atrosepticum; Bacteriophage; Myoviridae; Biocontrol; Cas; 4; CRISPR/CAS;
D O I
10.1007/s12230-019-09749-0
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Pectobacterium atrosepticum is a pectolytic enterobacterium that causes blackleg in plants. Bacteriophage Peat2 was isolated from P. atrosepticum infected potato tissue and its genome sequenced. Analysis of the 48,659 bp genome revealed that Peat2 resembles the Myoviridae family Klebsiella pneumoniae bacteriophage JD001 and includes a CRISPR Cas 4 RecB-like nuclease. A lytic bacteriophage, Peat2 details provide important information for application as a biocontrol agent of disease caused by phytopathogenic P. atrosepticum.
引用
收藏
页码:614 / 616
页数:3
相关论文
共 15 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   Evolution of Pectobacterium Bacteriophage ΦM1 To Escape Two Bifunctional Type III Toxin-Antitoxin and Abortive Infection Systems through Mutations in a Single Viral Gene [J].
Blower, Tim R. ;
Chai, Ray ;
Przybilski, Rita ;
Chindhy, Shahzad ;
Fang, Xinzhe ;
Kidman, Samuel E. ;
Tan, Hui ;
Luisi, Ben F. ;
Fineran, Peter C. ;
Salmond, George P. C. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2017, 83 (08)
[3]   Improved microbial gene identification with GLIMMER [J].
Delcher, AL ;
Harmon, D ;
Kasif, S ;
White, O ;
Salzberg, SL .
NUCLEIC ACIDS RESEARCH, 1999, 27 (23) :4636-4641
[4]  
Gill JJ, 2003, APPL MICROBIOL BIOT, V90, P1333
[5]   Genome Sequence of Pectobacterium carotovorum Phage PPWS1, Isolated from Japanese Horseradish [Eutrema japonicum (Miq.) Koidz] Showing Soft-Rot Symptoms [J].
Hirata, Hisae ;
Kashihara, Misako ;
Horiike, Tokumasa ;
Suzuki, Tomohiro ;
Dohra, Hideo ;
Netsu, Osamu ;
Tsuyumua, Shinji .
GENOME ANNOUNCEMENTS, 2016, 4 (02)
[6]   CRISPR/Cas, the Immune System of Bacteria and Archaea [J].
Horvath, Philippe ;
Barrangou, Rodolphe .
SCIENCE, 2010, 327 (5962) :167-170
[7]   Complete Genome Sequence of Phytopathogenic Pectobacterium atrosepticum Bacteriophage Peat1 [J].
Kalischuk, Melanie ;
Hachey, John ;
Kawchuk, Lawrence .
GENOME ANNOUNCEMENTS, 2015, 3 (04)
[8]   Complete Genome Sequence of Pectobacterium carotovorum subsp carotovorum Bacteriophage My1 [J].
Lee, Dong Hwan ;
Lee, Ju-Hoon ;
Shin, Hakdong ;
Ji, Samnyu ;
Roh, Eunjung ;
Jung, Kyusuk ;
Ryu, Sangryeol ;
Choi, Jaehyuk ;
Heu, Sunggi .
JOURNAL OF VIROLOGY, 2012, 86 (20) :11410-11411
[9]   Complete Genome Sequence of Phytopathogenic Pectobacterium carotovorum subsp carotovorum Bacteriophage PP1 [J].
Lee, Ju-Hoon ;
Shin, Hakdong ;
Ji, Samnyu ;
Malhotra, Shweta ;
Kumar, Mukesh ;
Ryu, Sangryeol ;
Heu, Sunggi .
JOURNAL OF VIROLOGY, 2012, 86 (16) :8899-8900
[10]   Complete genome sequence of the Pectobacterium carotovorum subsp carotovorum virulent bacteriophage PM1 [J].
Lim, Jeong-A ;
Shin, Hakdong ;
Lee, Dong Hwan ;
Han, Sang-Wook ;
Lee, Ju-Hoon ;
Ryu, Sangryeol ;
Heu, Sunggi .
ARCHIVES OF VIROLOGY, 2014, 159 (08) :2185-2187