Adsorption of DNA binding proteins to functionalized carbon nanotube surfaces with and without DNA wrapping

被引：2
|
作者：
Ishibashi, Yu [1 ]
Oura, Shusuke [1 ]
Umemura, Kazuo [1 ]
机构：
[1] Tokyo Univ Sci, Grad Sch Sci, Biophys Sect, Dept Phys,Shinjuku Ku, 1-3 Kagurazaka, Tokyo 1628601, Japan
来源：
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 2017年 / 46卷 / 06期
基金：
日本学术振兴会;
关键词：
DNA; Single-walled carbon nanotubes; DNA binding proteins; Atomic force microscopy; Polyethylene glycol; SINGLE-STRANDED-DNA; SENSING PLATFORM; FORCE MICROSCOPY; RECOGNITION; MOLECULES; HYBRIDS; WATER;
D O I：
10.1007/s00249-017-1200-3
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
We examined the adsorption of DNA binding proteins on functionalized, single-walled carbon nanotubes (SWNTs). When SWNTs were functionalized with polyethylene glycol (PEG-SWNT), moderate adsorption of protein molecules was observed. In contrast, nanotubes functionalized with CONH2 groups (CONH2-SWNT) exhibited very strong interactions between the CONH2-SWNT and DNA binding proteins. Instead, when these SWNT surfaces were wrapped with DNA molecules (thymine 30-mers), protein binding was a little decreased. Our results revealed that DNA wrapped PEG-SWNT was one of the most promising candidates to realize DNA nanodevices involving protein reactions on DNA-SWNT surfaces. In addition, the DNA binding protein RecA was more adhesive than single-stranded DNA binding proteins to the functionalized SWNT surfaces.
引用
收藏
页码：541 / 547
页数：7
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