Physiological and molecular responses to drought stress in rubber tree (Hevea brasiliensis Muell. Arg.)

被引:60
作者
Wang, Li-feng [1 ]
机构
[1] Chinese Acad Trop Agr Sci, Danzhou Invest & Expt Stn Trop Crops, Rubber Res Inst, Minist Agr, Danzhou 571737, Hainan, Peoples R China
基金
中国国家自然科学基金;
关键词
Antioxidative enzyme; Drought; Hevea brasiliensis; Osmoregulation; Photosynthesis; Reactive oxygen species; OXIDATIVE STRESS; SUPEROXIDE-DISMUTASE; NUCLEOTIDE-SEQUENCE; ELECTRON-TRANSPORT; LIPID-PEROXIDATION; WATER; GENE; INHIBITION; METABOLISM; EXPRESSION;
D O I
10.1016/j.plaphy.2014.08.012
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plant drought stress response and tolerance are complex biological processes. In order to reveal the drought tolerance mechanism in rubber tree, physiological responses and expressions of genes involved in energy biosynthesis and reactive oxygen species (ROS) scavenging were systematically analyzed following drought stress treatment. Results showed that relative water content (RWC) in leaves was continuously decreased with the severity of drought stress. Wilting leaves were observed at 7 day without water (dww). Total chlorophyll content was increased at 1 dww, but decreased from 3 dww. However, the contents of malondialdehyde (MDA) and proline were significantly increased under drought stress. Peroxidase (POD) and superoxide dismutase (SOD) activities were markedly enhanced at 1 and 3 dww, respectively. Meanwhile, the soluble sugar content was constant under drought stress. These indicated that photosynthetic activity and membrane lipid integrity were quickly attenuated by drought stress in rubber tree, and osmoregulation participated in drought tolerance mechanism in rubber tree. Expressions of energy biosynthesis and ROS scavenging systems related genes, including HbCuZnSOD, HbMnSOD, HbAPX, HbCAT, HbCOA, HbATP, and HbACAT demonstrated that these genes were significantly up-regulated by drought stress, and reached a maximum at 3 dww, then followed by a decrease from 5 dww. These results suggested that drought stress adaption in rubber tree was governed by energy biosynthesis, antioxidative enzymes, and osmoregulation. 0 2014 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:243 / 249
页数:7
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