Buspirone-induced changes in the serotonergic and non-serotonergic cells in the dorsal raphe nucleus of rats

被引：14

作者：

Jahanshahi, Ali ^{[1
,2
]}

Lim, Lee Wei ^{[1
,2
]}

Steinbusch, Harry W. M. ^{[1
]}

Visser-Vandewalle, Veerle ^{[2
]}

Temel, Yasin ^{[1
,2
]}

机构：

[1] Maastricht Univ, Dept Neurosci, Med Ctr, NL-6229 ER Maastricht, Netherlands

[2] Maastricht Univ, Dept Neurosurg, Med Ctr, NL-6229 ER Maastricht, Netherlands

来源：

NEUROSCIENCE LETTERS | 2010年 / 473卷 / 02期

关键词：

Buspirone; Anxiety; Serotonin (5-HT); Tyrosine hydroxylase (TH); Neuronal nitric oxide synthase (nNOS); NITRIC-OXIDE SYNTHASE; ALPHA-ADRENOCEPTOR ANTAGONISTS; GENERALIZED ANXIETY DISORDER; NADPH-DIAPHORASE; FIRING ACTIVITY; TYROSINE HYDROXYLATION; ACETYLCHOLINE-RELEASE; NERVOUS-SYSTEM; LOCUS-CERULEUS; FRONTAL-CORTEX;

D O I：

10.1016/j.neulet.2010.02.038

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Buspirone, a 5-HT (5-hydroxytryptamine, serotonin)(1A) partial agonist, is being used as an anxiolytic drug. The mechanism of action is explained by an effect on the 5-HT system. The main source of 5-HT in the forebrain is the dorsal raphe nucleus (DRN). However, there are also other populations of non-5-HT neurons in the DRN. Here, we investigated the effect of acute and chronic buspirone treatments on the 5-HT and non-5-HT cells, the neuronal nitric oxide synthase (nNOS) and tyrosine hydroxylase (TH) cells, in the DRN. Rats received either an acute or chronic administration of buspirone or saline. Hereafter, the brains were processed for 5-HT, nNOS, and TH immunohistochemistry. We found that acute and chronic buspirone treatments significantly lowered the mean optical density of nNOS in the DRN as compared to controls. Meanwhile only the chronic buspirone treatment reduced the mean density of 5-HT and TH immunoreactivity but not the acute buspirone as compared to saline treated animals. Our findings suggest that buspirone treatment affects not only the intracellular content of 5-HT but also nNOS and TH. Therefore, the cellular effect of buspirone is more complex than thought. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

引用

页码：136 / 140

页数：5

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