Neuroligin 1 regulates spines and synaptic plasticity via LIMK1/cofilin-mediated actin reorganization

被引：80

作者：

Liu, An ^{[1
]}

Zhou, Zikai ^{[1
,2
]}

Dang, Rui ^{[1
]}

Zhu, Yuehua ^{[1
]}

Qi, Junxia ^{[1
]}

He, Guiqin ^{[1
]}

Leung, Celeste ^{[3
,4
]}

Pak, Daniel ^{[5
]}

Jia, Zhengping ^{[3
,4
]}

Xie, Wei ^{[1
,2
]}

机构：

[1] Southeast Univ, Key Lab Dev Genes & Human Dis, Jiangsu Coinnovat Ctr Neuroregenerat, Nanjing 210096, Jiangsu, Peoples R China

[2] Southeast Univ, Collaborat Innovat Ctr Brain Sci, Inst Life Sci, Nanjing 210096, Jiangsu, Peoples R China

[3] Hosp Sick Children, Neurosci & Mental Hlth, 555 Univ Ave, Toronto, ON M5G 1X8, Canada

[4] Univ Toronto, Dept Physiol, Fac Med, Toronto, ON M5S 1A8, Canada

[5] Georgetown Univ, Med Ctr, Dept Pharmacol & Physiol, Washington, DC 20007 USA

来源：

JOURNAL OF CELL BIOLOGY | 2016年 / 212卷 / 04期

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院; 中国国家自然科学基金;

关键词：

AMPA RECEPTOR TRAFFICKING; CELL-ADHESION; ALPHA-NEUREXINS; LIM-KINASE; RAP GTPASE; COFILIN; ADF/COFILIN; ACTIVATION; DYNAMICS; SYNAPSES;

D O I：

10.1083/jcb.201509023

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Neuroligin (NLG) 1 is important for synapse development and function, but the underlying mechanisms remain unclear. It is known that at least some aspects of NLG1 function are independent of the presynaptic neurexin, suggesting that the C-terminal domain (CTD) of NLG1 may be sufficient for synaptic regulation. In addition, NLG1 is subjected to activity- dependent proteolytic cleavage, generating a cytosolic CTD fragment, but the significance of this process remains unknown. In this study, we show that the CTD of NLG1 is sufficient to (a) enhance spine and synapse number, (b) modulate synaptic plasticity, and (c) exert these effects via its interaction with spine-associated Rap guanosine triphosphatase-activating protein and subsequent activation of LIM-domain protein kinase 1/cofilin-mediated actin reorganization. Our results provide a novel postsynaptic mechanism by which NLG1 regulates synapse development and function.

引用

页码：449 / 463

页数：15

共 64 条

[1] Phosphorylation of cofilin by LIM-kinase is necessary for semaphorin 3A-induced growth cone collapse [J].