The effect of platelet-rich fibrin on normal dermal fibroblast proliferation after mitomycin-c treatment: An in vitro study

Background: Disturbance in the wound healing can cause the wound turn into a chronic wound, which histologically shows fibroblast senescence with weak proliferation ability. Mitomycin-C could block cell proliferation that causes cell senescence which is similar to the chronic wound morphology. Platelet-Rich Fibrin (PRF) contains a large number of platelets, leukocytes, cytokines and growth factors. This study aims to determine whether PRF could improve the fibroblast proliferation after treatment with Mitomycin-C. Methods: Cultured normal human skin fibroblasts forth passage divided into five groups. The first group was treated with culture medium, and the second group with 10 mu g/mL mitomycin-C for 2 h. The third, 4th and 5th group were treated with mitomycin-C for the same dose and period, then adding it with 100%, 50%, and 25% of PRF. The fibroblast proliferation was measured by MTT assay. Results: The fibroblast proliferation in the group with culture medium is 11.366,56 +/- 4.073,32, meanwhile in the group with mitomycin-C treatment is 5.690,41 +/- 2.834,22. The fibroblast proliferation in group with 100% PRF is 7.909,8 +/- 3.392,19; group with 50% PRF 15.347,91 +/- 8.413,02; and group with 25% PRF 13.449,56 +/- 7.523,83. All of the PRF groups increased significantly compared to the group with Mitomycin-C treatment. Conclusions: Platelet-Rich Fibrin can improve normal dermal fibroblast proliferation after treatment with mitomycin-C in vitro.