Short-chain fatty acids stimulate colonic transit via intraluminal 5-HT release in rats

被引:336
作者
Fukumoto, S
Tatewaki, M
Yamada, T
Fujimiya, M
Mantyh, C
Voss, M
Eubanks, S
Harris, M
Pappas, TN
Takahashi, T
机构
[1] Duke Univ, Med Ctr, Dept Surg, Durham, NC 27710 USA
[2] Shiga Univ Med Sci, Dept Anat, Shiga 52021, Japan
关键词
5-hydroxytryptamine(3) receptor; migrating contractions; vagovagal reflex;
D O I
10.1152/ajpregu.00442.2002
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
We studied whether physiological concentration of short-chain fatty acids (SCFAs) affects colonic transit and colonic motility in conscious rats. Intraluminal administration of SCFAs (100-200 mM) into the proximal colon significantly accelerated colonic transit. The stimulatory effect of SCFAs on colonic transit was abolished by perivagal capsaicin treatment, atropine, hexamethonium, and vagotomy, but not by guanethidine. The stimulatory effect of SCFAs on colonic transit was also abolished by intraluminal pretreatment with lidocaine and a 5-hydroxytryptamine (HT)(3) receptor antagonist. Intraluminal administration of SCFAs provoked contractions at the proximal colon, which migrated to the mid- and distal colon. SCFAs caused a significant increase in the luminal concentration of 5-HT of the vascularly isolated and luminally perfused rat colon ex vivo. It is suggested that the release of 5-HT from enterochromaffin cells in response to SCFAs stimulates 5-HT3 receptors located on the vagal sensory fibers. The sensory information is transferred to the vagal efferent and stimulates the release of acetylcholine from the colonic myenteric plexus, resulting in muscle contraction.
引用
收藏
页码:R1269 / R1276
页数:8
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