Engineering light-initiated afterglow lateral flow immunoassay for infectious disease diagnostics

被引：50

作者：

Hao, Liangwen ^{[1
]}

Yang, Weitao ^{[1
]}

Xu, Yan ^{[1
]}

Cui, Tianming ^{[1
]}

Zhu, Guoqi ^{[1
]}

Zeng, Weiwei ^{[1
]}

Bian, Kexin ^{[1
]}

Liang, Hongying ^{[1
]}

Zhang, Pengfei ^{[2
]}

Zhang, Bingbo ^{[1
]}

机构：

[1] Tongji Univ Canc Ctr, Tongji Univ, Shanghai Peoples Hosp 10, Inst Biomed Engn,Dept Med Ultrasound,Shanghai Fron, Shanghai 200072, Peoples R China

[2] Tongji Univ, Shanghai Skin Dis Hosp, Sch Med, Dept Cent Lab, Shanghai 200443, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2022年 / 212卷

基金：

中国国家自然科学基金;

关键词：

Infectious disease; Lateral flow immunoassay; Afterglow; HIV; SARS-CoV-2; NANOPARTICLES; SENSITIVITY; SARS-COV-2; AMPLIFICATION; ENHANCE; ASSAYS;

D O I：

10.1016/j.bios.2022.114411

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The pandemic of highly contagious diseases has put forward urgent requirements for high sensitivity and adaptive capacity of point-of-care testing (POCT). Herein, for the first time, we report an aggregation-induced emission (AIE) dye-energized light-initiated afterglow nanoprobes (named LiAGNPs), implemented onto a lateral flow immunoassay (LFIA) test strip, for diagnosis of two highly contagious diseases, human immunodeficiency virus (HIV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as model validation. The primary working mechanism relies on the cyclically generated singlet oxygen (O-1(2))-triggered time-resolved luminescent signals of LiAGNPs in which AIE dyes (TTMN) and chemiluminescent substrates (SO) are loaded. The designed LiAGNPs were found 2-fold and 32-fold sensitive than the currently used Eu(III)-based time resolved fluorescent nanoparticles and gold nanoparticles in lateral flow immunoassay (LFIA), respectively. In addition, the extra optical behaviors of nude color and fluorescence of LiAGNPs enable the LFIA platform with the capability of the naked eye and fluorescent detection to satisfy the applications under varying scenarios. In short, the versatile LiAGNPs have great potential as a novel time-resolved reporter in enhancing detection sensitivity and application flexibility with LFIA platform for rapid but sensitive infectious disease diagnostics.

引用

页数：11

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